Phosphoribosyl pyrophosphate (PRPP) pentasodium is an important metabolite required for the biosynthesis of purine and pyrimidine nucleotides, the amino acid (AA)s histidine and tryptophan, and the cofactors NAD and NADP.

Physicochemical Properties

Molecular Formula	C5H8NA5O14P3
Molecular Weight	499.98
Exact Mass	477.879
CAS #	108321-05-7
PubChem CID	92043170
Appearance	White to off-white solid powder
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	14
Rotatable Bond Count	6
Heavy Atom Count	26
Complexity	499
Defined Atom Stereocenter Count	3
SMILES	O=P([O-])([O-])OC[C@H]1O[C@H](OP(OP(O)(O)=O)(O)=O)[C@H](O)[C@@H]1O.[Na+].[Na+]
InChi Key	QZAPPJHVNYCTAX-CKJQBBATSA-J
InChi Code	InChI=1S/C5H13O14P3.4Na/c6-3-2(1-16-20(8,9)10)17-5(4(3)7)18-22(14,15)19-21(11,12)13;;;;/h2-7H,1H2,(H,14,15)(H2,8,9,10)(H2,11,12,13);;;;/q;4*+1/p-4/t2-,3-,4-,5?;;;;/m1..../s1
Chemical Name	tetrasodium;[[(3R,4S,5R)-3,4-dihydroxy-5-[[hydroxy(oxido)phosphoryl]oxymethyl]oxolan-2-yl]oxy-oxidophosphoryl] phosphate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	Human Endogenous Metabolite
ln Vitro	An essential step in cellular metabolism is phosphoribosyl pyrophosphate (PRPP) pentasodium. The following is the process by which PRPP synthase makes PRPP: ribose 5-phosphate + ATP → PRPP + AMP. Living things contain PRPP in large quantities. It is utilized in substitution processes to create glycosidic linkages. The amino acids histidine and tryptophan, the cofactors NAD and tetrahydromethotrexate, arabinose monophosphate, and some aminoglycoside antibiotics are all biosynthesised via PRPP [1]. In human erythrocytes without hypoxanthine-guanine phosphoribosyltransferase accumulation, phosphoribosyl diphosphate (5-Phosphoribosyl-l-pyrophosphate) pentasodium is a substrate shared by adenine phosphoribosyltransferase and hypoxanthine-guanine phosphoribosyltransferase. Purified adenine phosphoribosyltransferase is stabilized against thermal inactivation by adding 5-phosphoribosyl-l-pyrophosphate [2].
References	[1]. The importance of the five phosphoribosyl-pyrophosphate synthetase (Prs) gene products of Saccharomyces cerevisiae in the maintenance of cell integrity and the subcellular localization of Prs1p. Microbiology (Reading). 2000;146 Pt 12:3269-3278. [2]. Substrate stabilization: genetically controlled reciprocal relationship of two human enzymes. Science. 1970;167(3919):887-889.

Solubility Data

Solubility (In Vitro)	H2O: 50 mg/mL (100.00 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 25 mg/mL (50.00 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with heating and sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.0001 mL	10.0004 mL	20.0008 mL
	5 mM	0.4000 mL	2.0001 mL	4.0002 mL
	10 mM	0.2000 mL	1.0000 mL	2.0001 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.