Phosphonoacetic acid is an endogenously produced metabolite. Phosphonoacetic acid also has anti-orthopoxvirus activity.

Physicochemical Properties

Molecular Formula	C2H5O5P
Molecular Weight	140.03
Exact Mass	139.987
CAS #	4408-78-0
PubChem CID	546
Appearance	White to off-white solid powder
Density	1.858g/cm3
Boiling Point	490.2ºC at 760mmHg
Melting Point	143-146 °C(lit.)
LogP	-2
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	2
Heavy Atom Count	8
Complexity	133
Defined Atom Stereocenter Count	0
InChi Key	XUYJLQHKOGNDPB-UHFFFAOYSA-N
InChi Code	InChI=1S/C2H5O5P/c3-2(4)1-8(5,6)7/h1H2,(H,3,4)(H2,5,6,7)
Chemical Name	2-phosphonoacetic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	Human Endogenous Metabolite
References	[1]. A review of compounds exhibiting anti-orthopoxvirus activity in animal models. Antiviral Res. 2003 Jan;57(1-2):41-52.
Additional Infomation	Phosphonoacetic acid is a member of the class of phosphonic acids that is phosphonic acid in which the hydrogen attached to the phosphorous is replaced by a carboxymethyl group. It has a role as an antiviral agent and an EC 2.7.7.7 (DNA-directed DNA polymerase) inhibitor. It is a monocarboxylic acid and a member of phosphonic acids. It is functionally related to a phosphonic acid and an acetic acid. It is a conjugate acid of a hydrogen phosphonatoacetate(2-). Phosphonoacetate is a metabolite found in or produced by Escherichia coli (strain K12, MG1655). Fosfonet Sodium is a sodium salt formulation of phosphonoacetic acid with activity against herpes simplex viruses and other members of the herpes virus family. Fosfonet inhibits viral DNA polymerase and inhibits the synthesis of viral DNA. Phosphonoacetic Acid is an antiviral agent with activity against herpes simplex viruses and other members of the herpes virus family. Phosphonoacetic acid inhibits viral DNA polymerase and inhibits the synthesis of viral DNA. A simple organophosphorus compound that inhibits DNA polymerase, especially in viruses and is used as an antiviral agent.

Solubility Data

Solubility (In Vitro)	H2O : 260 mg/mL (1856.74 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 100 mg/mL (714.13 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	7.1413 mL	35.7066 mL	71.4133 mL
	5 mM	1.4283 mL	7.1413 mL	14.2827 mL
	10 mM	0.7141 mL	3.5707 mL	7.1413 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.