Peptide 74 is a synthetic peptide containing the prodomain sequence of a matrix metalloproteinase (MMP). Peptide 74 inhibits the activated form of 72-kDa type IV collagenase in vitro.

Physicochemical Properties

Molecular Formula	C62H107N23O20S2
Molecular Weight	1558.78508
Exact Mass	1557.75
CAS #	132116-39-3
PubChem CID	164323
Appearance	White to off-white solid powder
LogP	-14
Hydrogen Bond Donor Count	23
Hydrogen Bond Acceptor Count	26
Rotatable Bond Count	49
Heavy Atom Count	107
Complexity	3200
Defined Atom Stereocenter Count	14
SMILES	N/C(=N/CCC[C@@H](C(N[C@H](C(N1CCC[C@H]1C(N[C@H](C(N[C@H](C(NCC(N[C@H](C(N1CCC[C@H]1C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(=O)O)CC(=O)N)=O)C)=O)C(C)C)=O)CC(=O)O)=O)=O)CC(=O)N)=O)=O)CS)=O)CCC/N=C(\N)/N)=O)=O)CCCCN)=O)NC([C@@H](NC([C@H]([C@H](O)C)N)=O)CCSC)=O)/N
InChi Key	DAZBILQQVFBVPE-XKKUQSFHSA-N
InChi Code	InChI=1S/C62H107N23O20S2/c1-29(2)47(57(101)74-30(3)48(92)81-38(60(104)105)25-43(65)88)83-53(97)36(26-45(90)91)80-55(99)41-16-11-22-85(41)59(103)37(24-42(64)87)75-44(89)27-73-49(93)39(28-106)82-51(95)33(14-9-20-72-62(69)70)77-54(98)40-15-10-21-84(40)58(102)35(12-6-7-18-63)79-50(94)32(13-8-19-71-61(67)68)76-52(96)34(17-23-107-5)78-56(100)46(66)31(4)86/h29-41,46-47,86,106H,6-28,63,66H2,1-5H3,(H2,64,87)(H2,65,88)(H,73,93)(H,74,101)(H,75,89)(H,76,96)(H,77,98)(H,78,100)(H,79,94)(H,80,99)(H,81,92)(H,82,95)(H,83,97)(H,90,91)(H,104,105)(H4,67,68,71)(H4,69,70,72)/t30-,31+,32-,33-,34-,35-,36-,37-,38-,39-,40-,41-,46-,47-/m0/s1
Chemical Name	(2S)-4-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-1-[(2S)-4-amino-2-[[2-[[(2R)-2-[[(2S)-2-[[(2S)-1-[(2S)-6-amino-2-[[(2S)-2-[[(2S)-2-[[(2S,3R)-2-amino-3-hydroxybutanoyl]amino]-4-methylsulfanylbutanoyl]amino]-5-(diaminomethylideneamino)pentanoyl]amino]hexanoyl]pyrrolidine-2-carbonyl]amino]-5-(diaminomethylideneamino)pentanoyl]amino]-3-sulfanylpropanoyl]amino]acetyl]amino]-4-oxobutanoyl]pyrrolidine-2-carbonyl]amino]-3-carboxypropanoyl]amino]-3-methylbutanoyl]amino]propanoyl]amino]-4-oxobutanoic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	A2058 and HT 1080 tumor cell invasion is 60–80% lessened by peptide 74 (30 μM)[1]. Peptide 74 (30 μM) did not block chemotaxis, cause cytotoxicity, or change the quantity of HT1080 and A2058 human tumor cells [1].
References	[1]. Inhibition of tumor cell invasion by a highly conserved peptide sequence from the matrix metalloproteinase enzyme prosegment. Cancer Res. 1992 Apr 15;52(8):2353-6. [2]. Functional role of matrix metalloproteinases (MMPs) in mammary epithelial cell development. J Cell Physiol. 2001 Jul;188(1):75-88.

Solubility Data

Solubility (In Vitro)	H2O : ~100 mg/mL (~64.15 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 100 mg/mL (64.15 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	0.6415 mL	3.2076 mL	6.4152 mL
	5 mM	0.1283 mL	0.6415 mL	1.2830 mL
	10 mM	0.0642 mL	0.3208 mL	0.6415 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.