NSC 15364 is a VDAC1 oligomerization and apoptosis inhibitor.

Physicochemical Properties

Molecular Formula	C₁₃H₁₄N₄O
Molecular Weight	242.28
Exact Mass	242.116
CAS #	4550-72-5
PubChem CID	225703
Appearance	Gray to brown solid powder
Density	1.4±0.1 g/cm3
Boiling Point	381.4±27.0 °C at 760 mmHg
Flash Point	184.5±23.7 °C
Vapour Pressure	0.0±0.9 mmHg at 25°C
Index of Refraction	1.785
LogP	-0.5
Hydrogen Bond Donor Count	4
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	2
Heavy Atom Count	18
Complexity	242
Defined Atom Stereocenter Count	0
InChi Key	MAPWYRGGJSHAAU-UHFFFAOYSA-N
InChi Code	InChI=1S/C13H14N4O/c14-9-1-5-11(6-2-9)16-13(18)17-12-7-3-10(15)4-8-12/h1-8H,14-15H2,(H2,16,17,18)
Chemical Name	1,3-bis(4-aminophenyl)urea
Synonyms	NSC 15364 NSC 15364
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	One of the main targets of medications meant to control cells is the oligomerization of mitochondrial membrane protein potential channel 1 (VDAC1) [1].
References	[1]. Novel Compounds Targeting the Mitochondrial Protein VDAC1 Inhibit Apoptosis and Protect against Mitochondrial Dysfunction. J Biol Chem. 2016 Nov 25;291(48):24986-25003.

Solubility Data

Solubility (In Vitro)

DMSO : ~50 mg/mL (~206.37 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (10.32 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (8.59 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.1275 mL	20.6373 mL	41.2746 mL
	5 mM	0.8255 mL	4.1275 mL	8.2549 mL
	10 mM	0.4127 mL	2.0637 mL	4.1275 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.