NMDAR/TRPM4-IN-2 (compound 8) is a potent inhibitor of the NMDAR/TRPM4 interaction interface. NMDAR/TRPM4-IN-2 has neuro-protection activity. NMDAR/TRPM4-IN-2 prevents NMDA-induced cell death and mitochondrial dysfunction in hippocampal neurons with IC50 of 2.1 μM. NMDAR/TRPM4-IN-2 protects mice from MCAO-induced brain injury and NMDA-induced retinal ganglion cell loss.

Physicochemical Properties

Molecular Formula	C11H18BRCLN2
Molecular Weight	293.631021022797
Exact Mass	328.01
CAS #	2243506-33-2
PubChem CID	138040845
Appearance	White to yellow solid powder
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	5
Heavy Atom Count	16
Complexity	152
Defined Atom Stereocenter Count	0
SMILES	C(C1C=CC=C(Br)C=1)N(CC)CCN.Cl
InChi Key	AQDSZKTVQVSVNC-UHFFFAOYSA-N
InChi Code	InChI=1S/C11H17BrN2.2ClH/c1-2-14(7-6-13)9-10-4-3-5-11(12)8-10;;/h3-5,8H,2,6-7,9,13H2,1H3;2*1H
Chemical Name	N'-[(3-bromophenyl)methyl]-N'-ethylethane-1,2-diamine;dihydrochloride
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	NMDAR/TRPM4-IN-2 (compound 8) (0-10 μM) decreases the interactions of GluN2A and GluN2B with TRPM4 in a dose-dependent manner[1]. NMDAR/TRPM4-IN-2 removes the CREB shutdown pathway and restores ERK1/2 activation and IEG induction while sparing the synaptic activity-driven, transcription-promoting functions of NMDARs[1].
References	[1]. Coupling of NMDA receptors and TRPM4 guides discovery of unconventional neuroprotectants. Science. 2020 Oct 9;370(6513):eaay3302.

Solubility Data

Solubility (In Vitro)

DMSO: 250 mg/mL (757.37 mM) H2O: 100 mg/mL (302.95 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (6.30 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (6.30 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.08 mg/mL (6.30 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.4056 mL	17.0282 mL	34.0565 mL
	5 mM	0.6811 mL	3.4056 mL	6.8113 mL
	10 mM	0.3406 mL	1.7028 mL	3.4056 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.