Physicochemical Properties
| Molecular Formula | C15H12N6O2S3 |
| Molecular Weight | 404.489777565002 |
| Exact Mass | 404.018 |
| CAS # | 1222765-97-0 |
| PubChem CID | 44142148 |
| Appearance | White to light yellow solid powder |
| LogP | 2.8 |
| Hydrogen Bond Donor Count | 0 |
| Hydrogen Bond Acceptor Count | 9 |
| Rotatable Bond Count | 4 |
| Heavy Atom Count | 26 |
| Complexity | 603 |
| Defined Atom Stereocenter Count | 0 |
| SMILES | S(C)(C1C=CC=C(C=1)C1=CSC2C1=C(N=CN=2)SC1=NN=NN1C)(=O)=O |
| InChi Key | IIEFAIOSYLQBJX-UHFFFAOYSA-N |
| InChi Code | InChI=1S/C15H12N6O2S3/c1-21-15(18-19-20-21)25-14-12-11(7-24-13(12)16-8-17-14)9-4-3-5-10(6-9)26(2,22)23/h3-8H,1-2H3 |
| Chemical Name | 5-(3-methylsulfonylphenyl)-4-(1-methyltetrazol-5-yl)sulfanylthieno[2,3-d]pyrimidine |
| Synonyms | NCT504; NCT 504 |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| ln Vitro | NCT-504 does not reduce PIP4Kγ's intrinsic ATP hydrolytic activity when PI5P substrate is not present[1]. NCT-504 modestly inhibits PIP4Kalpha phosphorylation of PI5P but does not inhibit PIP4Kbeta [1]. PIP4Kbeta and PIP4Kalpha are not inhibited by NCT-504 at 50 μM (IC50 between 50 μM and 100 μM) [1]. In MEFs, NCT-504 raises the amounts of PI(3,5)P2, PI3P, and PI5P [1]. In MEFs, NCT-504 (10 μM; 12 hours) has no effect on cell viability [1]. The autophagic cargo turnover rate and activation of autophagy are increased by NCT-504 (5 μM, 10 μM; 2 hours, 6 hours) [1]. Autolysosome formation is significantly increased with NCT-504 administration, while autophagosome formation is only slightly increased [1]. In 293A cells, NCT-504 reduces huntingtin and promotes autophagic flux [1]. In immortalized striatal cells from knock-in HD mice, NCT-504 lowers the levels of the mHtt protein [1]. |
| Cell Assay |
Western Blot Analysis[1] Cell Types: HEK293T Cell Tested Concentrations: 5 μM, 10 μM Incubation Duration: 2 hrs (hours), 6 hrs (hours) Experimental Results: Induction of autophagosome formation. |
| References |
[1]. Inhibition of PIP4Kγ Ameliorates the Pathological Effects of Mutant Huntingtin Protein. Elife. 2017 Dec 26;6:e29123. |
| Additional Infomation | 5-(3-methylsulfonylphenyl)-4-[(1-methyl-5-tetrazolyl)thio]thieno[2,3-d]pyrimidine is an aryl sulfide and a thienopyrimidine. |
Solubility Data
| Solubility (In Vitro) | DMSO : ~125 mg/mL (~309.03 mM) |
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples. Injection Formulations (e.g. IP/IV/IM/SC) Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] *Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline) Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline) Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline) Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil) Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Oral Formulations Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). Oral Formulation 3: Dissolved in PEG400 Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose Oral Formulation 6: Mixing with food powders Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4722 mL | 12.3612 mL | 24.7225 mL | |
| 5 mM | 0.4944 mL | 2.4722 mL | 4.9445 mL | |
| 10 mM | 0.2472 mL | 1.2361 mL | 2.4722 mL |