Medicagenic acid (Castanogenin) is extracted from the roots of Herniaria glabra and has potent bacteriostatic effects against plant pathogens and human dermatophytes. Medicagenic acid (Castanogenin) has low enzyme inhibitory activity. The target enzymes are xanthine oxidase, collagenase, elastase, tyrosinase, and ChE.

Physicochemical Properties

Molecular Formula	C30H46O6
Molecular Weight	502.68
Exact Mass	502.329
CAS #	599-07-5
PubChem CID	65048
Appearance	White to off-white solid powder
Density	1.23g/cm3
Boiling Point	635.9ºC at 760 mmHg
Melting Point	380-382ºC (dec.)
Flash Point	352.4ºC
Index of Refraction	1.587
LogP	5.269
Hydrogen Bond Donor Count	4
Hydrogen Bond Acceptor Count	6
Rotatable Bond Count	2
Heavy Atom Count	36
Complexity	1020
Defined Atom Stereocenter Count	10
SMILES	C[C@@]12CC[C@@H]3[C@@]([C@H]1CC=C4[C@]2(CC[C@@]5([C@H]4CC(CC5)(C)C)C(=O)O)C)(C[C@@H]([C@@H]([C@@]3(C)C(=O)O)O)O)C
InChi Key	IDGXIXSKISLYAC-WNTKNEGGSA-N
InChi Code	InChI=1S/C30H46O6/c1-25(2)11-13-30(24(35)36)14-12-27(4)17(18(30)15-25)7-8-20-26(3)16-19(31)22(32)29(6,23(33)34)21(26)9-10-28(20,27)5/h7,18-22,31-32H,8-16H2,1-6H3,(H,33,34)(H,35,36)/t18-,19-,20+,21+,22-,26+,27+,28+,29-,30-/m0/s1
Chemical Name	(2S,3R,4S,4aR,6aR,6bS,8aS,12aS,14aR,14bR)-2,3-dihydroxy-4,6a,6b,11,11,14b-hexamethyl-1,2,3,4a,5,6,7,8,9,10,12,12a,14,14a-tetradecahydropicene-4,8a-dicarboxylic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. Saponins as antimycotic agents: glycosides of medicagenic acid. Adv Exp Med Biol. 1996;404:535-46. [2]. https://www.ncbi.nlm.nih.gov/pubmed/31627115.
Additional Infomation	Medicagenic acid is a triterpenoid. Medicagenic acid has been reported in Medicago blancheana, Medicago granadensis, and other organisms with data available.

Solubility Data

Solubility (In Vitro)

DMSO : 100 mg/mL (198.93 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 2.5 mg/mL (4.97 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (4.97 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (4.97 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.9893 mL	9.9467 mL	19.8934 mL
	5 mM	0.3979 mL	1.9893 mL	3.9787 mL
	10 mM	0.1989 mL	0.9947 mL	1.9893 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.