MQA-P is a versatile near-infrared (NIR) fluorescent probe that can simultaneously detect ONOO-, viscosity, and polarity within mitochondria. MQA-P exhibits a significant response to ONOO- at λem=645 nm; and is highly sensitive to viscosity/polarity in the NIR channel at λem>704 nm. MQA-P has excited-state intramolecular charge transfer (ESICT) properties, and is highly sensitive to polarity by designing N,N-dimethylamino groups as electron donors and quinoline cation units as electron acceptors. MQA-P is used for ferroptosis or cancer diagnosis in vitro and in vivo through dual-channel images.

Physicochemical Properties

Molecular Formula	C42H36F3N2O4P
Molecular Weight	720.72
Appearance	Solid powder
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Dissolve MQA-P in dimethyl sulfoxide (DMSO) to prepare a stock solution (1.0 mM). 2. For imaging of ONOO- in live cells. HeLa cells were incubated with MQA-P (5 μM) for 30 min as a control; pretreated with SIN-1 (HY-126849; 100 μM) for 30 min, and then incubated with MQA-P (5 μM) for another 30 min. Green channel (λex=405 nm, λem=550-670 nm). 3. Live cell viscosity imaging. HeLa cells were incubated with MQA-P (5 μM) for 30 min as a control; pretreated with monensin (HY-N4302; 10 μM) for 30 min, and then incubated with MQA-P (5 μM) for 30 min. Fluorescence images were obtained using a confocal laser scanning microscope, with the red channel (λex=561 nm, λem=680-750 nm). 4. Dual-channel imaging of ONOO-, viscosity, and polarity during ferroptosis. HeLa cells were incubated with MQA-P (5 μM) for 30 min as a control; they were pretreated with Erastin (HY-15763; 50 μM) for 30 min and then incubated with MQA-P (5 μM) for another 30 min. Fluorescence images were obtained using a confocal laser scanning microscope, with the green channel (λex=405nm, λem=550-670 nm) for ONOO- and the red channel (λex=561 nm, λem=680-750 nm) for viscosity and polarity [1].
ln Vivo	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. For tissue section imaging, normal organs (including heart, liver, spleen, lung, and kidney) and tumors were isolated from mice and then cut into 5 μm thick sections. 2. These sections were incubated with MQA-P (20 μM) for 30 minutes, then washed three times with PBS (pH 7.4) and imaged in vivo using a confocal laser scanning microscope, using the green channel (λex=405 nm, λem=550-670 nm) for ONOO- and the red channel (λex=561 nm, λem=680-750 nm) for viscosity and polarity [1].
References	[1]. Multifunctional Fluorescent Probe for Simultaneous Detection of ONOO-, Viscosity, and Polarity and Its Application in Ferroptosis and Cancer Models. Anal Chem. 2023 Apr 4;95(13):5780-5787.

Solubility Data

Solubility (In Vitro)

DMSO : 10 mg/mL (13.88 mM; with sonication)

Solubility (In Vivo)

Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples. Injection Formulations (e.g. IP/IV/IM/SC) Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] *Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline) Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline) Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline) Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil) Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Oral Formulations Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). Oral Formulation 3: Dissolved in PEG400 Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose Oral Formulation 6: Mixing with food powders Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.3875 mL	6.9375 mL	13.8750 mL
	5 mM	0.2775 mL	1.3875 mL	2.7750 mL
	10 mM	0.1388 mL	0.6938 mL	1.3875 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.