MMG-11 quarterhydrate is a potent and specific human TLR2 antagonist. MMG-11 quarterhydrate inhibits TLR2/1 and TLR2/6 signal transduction, inhibits Pam3CSK4-induced hTLR2/1 with IC50 of 1.7 μM, and inhibits Pam2CSK4-induced hTLR2/6 response with IC50 of 5.7 μM.

Physicochemical Properties

Molecular Formula	C15H14O7.1/4H2O
Molecular Weight	310.78
Related CAS #	MMG-11;313254-94-3
Appearance	Off-white to light yellow solid powder
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	TLR2
ln Vitro	MMG-11 does not exhibit cellular toxicity or interfere with signaling that is triggered by TNF, IL-1β, or other TLR agonists. Up to 100 µM, MMG-11 (0.01-100 µM) does not exhibit any cytotoxic effects on peripheral blood mononuclear cells (PBMCs)[1].
Cell Assay	Cell Cytotoxicity Assay[1] Cell Types: PBMCs Tested Concentrations: 0.01, 1, 10, 100 µM Incubation Duration: Experimental Results: demonstrated no cytotoxic effects up to 100 µM.
References	[1]. Identification of a pyrogallol derivative as a potent and selective human TLR2 antagonist by structure-based virtual screening. Biochem Pharmacol. 2018 Aug;154:148-160.

Solubility Data

Solubility (In Vitro)

DMSO :~100 mg/mL (~321.77 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (8.04 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.04 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. Solubility in Formulation 3: ≥ 2.08 mg/mL (6.69 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.2177 mL	16.0886 mL	32.1771 mL
	5 mM	0.6435 mL	3.2177 mL	6.4354 mL
	10 mM	0.3218 mL	1.6089 mL	3.2177 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.