Ligustrazine hydrochloride (Chuanxiongzine HCl; Tetramethylpyrazine) is a natural product a natural product with protective effects against injured ECV304 cells. It can significantly increase NOS and NO formation compared with the model group.
Physicochemical Properties
| Molecular Formula | C₈H₁₂N₂ |
| Molecular Weight | 136.19 |
| Exact Mass | 172.076 |
| CAS # | 76494-51-4 |
| Related CAS # | Ligustrazine;1124-11-4 |
| PubChem CID | 156709 |
| Appearance | White to off-white solid powder |
| Boiling Point | 192.7ºC at 760mmHg |
| Flash Point | 71.6ºC |
| LogP | 2.512 |
| Hydrogen Bond Donor Count | 1 |
| Hydrogen Bond Acceptor Count | 2 |
| Rotatable Bond Count | 0 |
| Heavy Atom Count | 11 |
| Complexity | 87.8 |
| Defined Atom Stereocenter Count | 0 |
| InChi Key | RQKFOGXUTRDQPB-UHFFFAOYSA-N |
| InChi Code | InChI=1S/C8H12N2.ClH/c1-5-6(2)10-8(4)7(3)9-5;/h1-4H3;1H |
| Chemical Name | 2,3,5,6-tetramethylpyrazine;hydrochloride |
| Synonyms | Chuanxiongzine hydrochlorideChuanxiongzine HCl Tetramethylpyrazine |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| References |
[1]. Protective effect of ligustrazine hydrochloride on homocysteine-injured ECV304 cells. Zhongguo Zhong Yao Za Zhi. 2012 Jun;37(12):1836-9. [2]. Brain microdialysate, CSF and plasma pharmacokinetics of ligustrazine hydrochloride in rats after intranasal and intravenous administration. Biopharm Drug Dispos. 2013 Oct;34(7):417-22. |
| Additional Infomation | Ligustrazine HCl is a member of pyrazines. |
Solubility Data
| Solubility (In Vitro) |
DMSO : ~100 mg/mL H2O : ~100 mg/mL |
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (Infinity mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (Infinity mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (Infinity mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. Solubility in Formulation 4: 100 mg/mL (Infinity mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 7.3427 mL | 36.7134 mL | 73.4268 mL | |
| 5 mM | 1.4685 mL | 7.3427 mL | 14.6854 mL | |
| 10 mM | 0.7343 mL | 3.6713 mL | 7.3427 mL |