LSD1-IN-30 (compound 3) is a potent inhibitor of lysine-specific demethylase 1 (LSD1) with IC50 of 0.291 μM.

Physicochemical Properties

Molecular Formula	C15H14N2O3
Molecular Weight	270.283263683319
Exact Mass	270.1
CAS #	1289575-45-6
PubChem CID	135442596
Appearance	Off-white to yellow solid powder
LogP	2.3
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	3
Heavy Atom Count	20
Complexity	362
Defined Atom Stereocenter Count	0
SMILES	C/C(=N\NC(=O)C1=CC=C(C=C1)O)/C2=CC=CC=C2O
InChi Key	LVGDDYSLMCRYJE-MHWRWJLKSA-N
InChi Code	InChI=1S/C15H14N2O3/c1-10(13-4-2-3-5-14(13)19)16-17-15(20)11-6-8-12(18)9-7-11/h2-9,18-19H,1H3,(H,17,20)/b16-10+
Chemical Name	4-hydroxy-N-[(E)-1-(2-hydroxyphenyl)ethylideneamino]benzamide
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	LSD1 0.291 μM (IC50)
References	[1].High-Throughput Virtual Screening Identifies Novel N′‑(1-Phenylethylidene)-benzohydrazides as Potent, Specific, and Reversible LSD1 Inhibitors. J Med Chem . 2013 Dec 12;56(23):9496-508.

Solubility Data

Solubility (In Vitro)

DMSO :~100 mg/mL (~369.99 mM; with sonication)

Solubility (In Vivo)

Solubility in Formulation 1: 2.5 mg/mL (9.25 mM) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one),clear solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared,you can add 100 μL of 25.0 mg/mL clear DMSO stock solution and add it to 400 μL PEG300 and mix well. Then add 50 μL Tween-80 to the above system and mix well. Then continue to add 450 μL of physiological saline to make up to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (9.25 mM) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one),clear solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared,you can add 100 μL of 25.0 mg/mL clear DMSO stock solution and add it to 900 μL corn oil and mix well.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.6999 mL	18.4993 mL	36.9987 mL
	5 mM	0.7400 mL	3.6999 mL	7.3997 mL
	10 mM	0.3700 mL	1.8499 mL	3.6999 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.