L-Histidine HCl hydrate (H-His-OH.HCl.H2O) is an endogenously produced metabolite.

Physicochemical Properties

Molecular Formula	C6H12CLN3O3
Molecular Weight	209.63
Exact Mass	209.056
CAS #	5934-29-2
Related CAS #	L-Histidine-d5 hydrochloride hydrate;2483831-75-8;L-Histidine-15N hydrochloride hydrate;L-Histidine-13C hydrochloride hydrate;2483735-43-7;L-Histidine-13C6,15N3,d5 hydrochloride hydrate;2483829-32-7;L-Histidine-13C6 hydrochloride hydrate;201740-88-7;L-Histidine-13C6,15N3 hydrochloride hydrate;202468-43-7;L-Histidine-15N3 hydrochloride hydrate;L-Histidine-d3 hydrochloride hydrate;L-Histidine-15N3,d5 hydrochloride hydrate
PubChem CID	165377
Appearance	White to off-white solid powder
Density	1.485 g/cm3
Boiling Point	458.9ºC at 760 mmHg
Melting Point	254 °C (dec.)(lit.)
Flash Point	231.3ºC
LogP	0.802
Hydrogen Bond Donor Count	5
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	3
Heavy Atom Count	13
Complexity	151
Defined Atom Stereocenter Count	1
SMILES	C1=C(NC=N1)C[C@@H](C(=O)O)N.O.Cl
InChi Key	CMXXUDSWGMGYLZ-XRIGFGBMSA-N
InChi Code	InChI=1S/C6H9N3O2.ClH.H2O/c7-5(6(10)11)1-4-2-8-3-9-4;;/h2-3,5H,1,7H2,(H,8,9)(H,10,11);1H;1H2/t5-;;/m0../s1
Chemical Name	(2S)-2-amino-3-(1H-imidazol-5-yl)propanoic acid;hydrate;hydrochloride
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Solubility Data

Solubility (In Vitro)	H2O: 100 mg/mL (477.03 mM) DMSO: 1 mg/mL (4.77 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 50 mg/mL (238.52 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.7703 mL	23.8515 mL	47.7031 mL
	5 mM	0.9541 mL	4.7703 mL	9.5406 mL
	10 mM	0.4770 mL	2.3852 mL	4.7703 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.