HPV16 E7 (86-93) TFA is a human leukocyte antigen (HLA)-A2.1 restricted HPV16 E7-derived peptide. HPV16 E7 (86-93) TFA is immunogenic in cervical cancer.

Physicochemical Properties

Molecular Formula	C??H??F?N?O??S
Molecular Weight	929.06
Related CAS #	HPV16 E7 (86-93);160212-93-1
Appearance	Solid powder
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	HPV16 E7 (86-93) specific T cells can be expanded using IVS (in vitro stimulation) with cognate peptide-pulsed dendritic cells (DCs) and are reactive against peptide-pulsed targets or, in the instance of E711-20 epitope-specific T cells, against HPV16 E7 expressing CaSki cell lines[1]. The sole response against K562 cells pulsed with peptide HPV16 E7 (86-93) was considerably suppressed by anti-HLA class I Ab w6/32, although this was not the case for the HPV16 E7-expressing tumor cell line CaSki. Precursor T cells that recognize the HPV16 E7 (86-93) peptide can develop into HPV-specific effector cells, at least in vitro [1]. HPV16 E7 peptide-loaded autologous dendritic cells (DCs) can activate a particular cytotoxic CD8+ T-cell response[2].
ln Vivo	In IVS culture, the HPV16 E7 (86–93) peptide causes a particular proliferation of T cells. The T cells that are specific to HPV16 E7 (86–93) do not identify the naturally occurring HPV16 E7-expressing cell line CaSki. Similar findings have also been reported for HPV16 E7 (86–93) peptides in transgenic mice. If the HPV16 E7 (86–93) peptide is loaded on antigen-presenting HLA class I molecules, it can trigger cytotoxic T lymphocyte (CTL) responses; however, HPV16-infected cells do not appear to digest or present the peptide[1].
References	[1]. T cells specific for HPV16 E7 epitopes in patients with squamous cell carcinoma of the oropharynx. Int J Cancer. 2006 Apr 15;118(8):1984-91. [2]. Induction of human papillomavirus type 16-specific immunologic responses in a normal and an human papillomavirus-infected populations. Immunology. 2005 May;115(1):136-49.

Solubility Data

Solubility (In Vitro)

May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (2.69 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (2.69 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (2.69 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.0764 mL	5.3818 mL	10.7636 mL
	5 mM	0.2153 mL	1.0764 mL	2.1527 mL
	10 mM	0.1076 mL	0.5382 mL	1.0764 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.