HN37 is a potent and stable compound with anti-epileptic activity, with EC50 of 37 nM against KCNQ2.

Physicochemical Properties

Molecular Formula	C20H21FN2O2
Molecular Weight	340.39
Exact Mass	340.158
CAS #	1821222-10-9
PubChem CID	92045023
Appearance	Off-white to light yellow solid powder
LogP	4.1
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	6
Heavy Atom Count	25
Complexity	469
Defined Atom Stereocenter Count	0
SMILES	C1=C(C=C(C(NC(OC)=O)=C1C)C)N(CC#C)CC1C=CC(F)=CC=1
InChi Key	HXUBJZRAVCPBRH-UHFFFAOYSA-N
InChi Code	InChI=1S/C20H21FN2O2/c1-5-10-23(13-16-6-8-17(21)9-7-16)18-11-14(2)19(15(3)12-18)22-20(24)25-4/h1,6-9,11-12H,10,13H2,2-4H3,(H,22,24)
Chemical Name	methyl N-[4-[(4-fluorophenyl)methyl-prop-2-ynylamino]-2,6-dimethylphenyl]carbamate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Similar to RTG in terms of isoform selectivity, HN37 is a strong neuronal Kv7 activator [1].
References	[1]. Discovery of HN37 as a Potent and Chemically Stable Antiepileptic Drug Candidate. J Med Chem. 2021 May 13;64(9):5816-5837.

Solubility Data

Solubility (In Vitro)

DMSO : ~50 mg/mL (~146.89 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (7.34 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.9378 mL	14.6890 mL	29.3781 mL
	5 mM	0.5876 mL	2.9378 mL	5.8756 mL
	10 mM	0.2938 mL	1.4689 mL	2.9378 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.