HKSOX-1 is a fluorescent probe used to image and detect endogenous superoxide in living cells and in vivo. HKSOX-1 has excellent selectivity and sensitivity towards superoxide anion radicals.

Physicochemical Properties

Molecular Formula	C23H6F10O11S2
Molecular Weight	712.40
Appearance	Solid powder
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	In potassium phosphate buffer at 25 °C, HKSOX-1 (10 μM) combines with O2?-to produce a strong time-dependent fluorescence increase that lasts for 60 minutes without changing in intensity. The reaction is finished in 10 minutes. HKSOX-1 (10 μM) has remarkable stability against pH variations throughout the 2.2–8.8 range [1].
References	[1]. Fluorescent Probe HKSOX-1 for Imaging and Detection of Endogenous Superoxide in Live Cells and In Vivo. J Am Chem Soc. 2015 Jun 3;137(21):6837-43.

Solubility Data

Solubility (In Vitro)

DMSO :~110 mg/mL (~154.41 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 2.75 mg/mL (3.86 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 27.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.75 mg/mL (3.86 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 27.5 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.4037 mL	7.0185 mL	14.0371 mL
	5 mM	0.2807 mL	1.4037 mL	2.8074 mL
	10 mM	0.1404 mL	0.7019 mL	1.4037 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.