cis-4-Hydroxy-D-proline is a precursor for the synthesis of conformationally restricted PNA adenine monomers. cis-4-Hydroxy-D-proline may be utilized to study the specificity and kinetics of D-alanine dehydrogenase.

Physicochemical Properties

Molecular Formula	C5H9NO3
Molecular Weight	131.13
Exact Mass	131.058
CAS #	2584-71-6
PubChem CID	440014
Appearance	White to gray solid powder
Density	1.4±0.1 g/cm3
Boiling Point	355.2±42.0 °C at 760 mmHg
Melting Point	243 °C (dec.)(lit.)
Flash Point	168.6±27.9 °C
Vapour Pressure	0.0±1.8 mmHg at 25°C
Index of Refraction	1.540
LogP	-1.84
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	1
Heavy Atom Count	9
Complexity	125
Defined Atom Stereocenter Count	2
SMILES	C1[C@H](CN[C@H]1C(=O)O)O
InChi Key	PMMYEEVYMWASQN-QWWZWVQMSA-N
InChi Code	InChI=1S/C5H9NO3/c7-3-1-4(5(8)9)6-2-3/h3-4,6-7H,1-2H2,(H,8,9)/t3-,4-/m1/s1
Chemical Name	(2R,4R)-4-hydroxypyrrolidine-2-carboxylic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. Pyrrolidine PNA: a novel conformationally restricted PNA analogue. Org Lett. 2000 Dec 28;2(26):4161-3. [2]. Characterization of cis-4-hydroxy-D-proline dehydrogenase from Sinorhizobium meliloti. Biosci Biotechnol Biochem. 2018 Jan;82(1):110-113.
Additional Infomation	Cis-4-hydroxy-D-proline is a 4-hydroxy-D-proline in which the hydroxy group at position 4 has R-configuration. It has a role as a metabolite and a mouse metabolite. It is a tautomer of a cis-4-hydroxy-D-proline zwitterion. cis-4-Hydroxy-D-proline has been reported in Daphnia pulex and Aeromonas veronii with data available.

Solubility Data

Solubility (In Vitro)	H2O: 100 mg/mL (762.60 mM) DMSO: 1 mg/mL (7.63 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 100 mg/mL (762.60 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	7.6260 mL	38.1301 mL	76.2602 mL
	5 mM	1.5252 mL	7.6260 mL	15.2520 mL
	10 mM	0.7626 mL	3.8130 mL	7.6260 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.