GW 610 (NSC 721648) is an antineoplastic/anticancer agent that displays potent and specific anti-cancer activity against lung, colon, and breast cancer cell lines.

Physicochemical Properties

Molecular Formula	C15H12FNO2S
Molecular Weight	289.32
Exact Mass	289.057
CAS #	872726-44-8
PubChem CID	6712941
Appearance	Light yellow to brown solid powder
Density	1.3±0.1 g/cm3
Boiling Point	422.8±55.0 °C at 760 mmHg
Flash Point	209.5±31.5 °C
Vapour Pressure	0.0±1.0 mmHg at 25°C
Index of Refraction	1.619
LogP	4.7
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	3
Heavy Atom Count	20
Complexity	333
Defined Atom Stereocenter Count	0
SMILES	FC1C=C2C(SC(C3C=C(OC)C(OC)=CC=3)=N2)=CC=1
InChi Key	ZRLSVQBGBBYEAZ-UHFFFAOYSA-N
InChi Code	InChI=1S/C15H12FNO2S/c1-18-12-5-3-9(7-13(12)19-2)15-17-11-8-10(16)4-6-14(11)20-15/h3-8H,1-2H3
Chemical Name	2-(3,4-dimethoxyphenyl)-5-fluoro-1,3-benzothiazole
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	MCF-7, MDA 468, KM 12, and HCC 2998 cells are inhibited from proliferating by GW 610, with GI50s of <0.1 nM, <0.1 nM, 290 nM, and 0.25 nM, respectively[1]. In MDA-MB-468, MCF-7, KM12, and HCC2998 cells, GW 610 (0.1–1 μM) stimulates CYP1A1 mRNA and protein, but only in breast cancer cells does it induce CYP2S1 and CYP2W1[3].
Cell Assay	Western Blot Analysis[3] Cell Types: MDA- MB-468, MCF-7, KM12, and HCC2998 cells Tested Tested Concentrations: 0.1, 1 μM Incubation Duration: 48 hrs (hours) Experimental Results: Induced CYP1A1 mRNA and protein in all cell lines including KM12 and HCC2998 CRC cells. Induced CYP2S1 and CYP2W1 mRNA and protein expression in MDA-MB-468 and MCF-7 breast cancer cells.
References	[1]. Mortimer CG, et, al. Antitumor benzothiazoles. 26.(1) 2-(3,4-dimethoxyphenyl)-5-fluorobenzothiazole (GW 610, NSC 721648), a simple fluorinated 2-arylbenzothiazole, shows potent and selective inhibitory activity against lung, colon, and breast cancer cell [2]. Aiello S, et, al. Synthesis and biological properties of benzothiazole, benzoxazole, and chromen-4-one analogues of the potent antitumor agent 2-(3,4-dimethoxyphenyl)-5-fluorobenzothiazole (PMX 610, NSC 721648). J Med Chem. 2008 Aug 28;51(16):5135-9. [3]. Tan BS, et, al. CYP2S1 and CYP2W1 mediate 2-(3,4-dimethoxyphenyl)-5-fluorobenzothiazole (GW-610, NSC 721648) sensitivity in breast and colorectal cancer cells. Mol Cancer Ther. 2011 Oct;10(10):1982-92.

Solubility Data

Solubility (In Vitro)

DMSO: 25 mg/mL (86.41 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (8.64 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.4564 mL	17.2819 mL	34.5638 mL
	5 mM	0.6913 mL	3.4564 mL	6.9128 mL
	10 mM	0.3456 mL	1.7282 mL	3.4564 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.