GPR120 Agonist 2 is a potent and selective agonist of the GPR120 (G-protein coupled receptor) receptor. It is taken from example 209 of patent US 20110313003 A1. The human GPR120 gene encodes GPR 120, a protein belonging to the rhodopsin family. GPR120 has also been shown to mediate the anti-inflammatory and insulin-sensitizing effects of omega 3 fatty acids. Obesity results from decreased fat metabolism, which is caused by a lack of GPR120.

Physicochemical Properties

Molecular Formula	C22H25CLO4
Molecular Weight	388.89
Exact Mass	388.14
Elemental Analysis	C, 67.95; H, 6.48; Cl, 9.12; O, 16.46
CAS #	1234844-11-1
Related CAS #	1234844-11-1
PubChem CID	46700745
Appearance	White to off-white solid powder
LogP	5.1
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	6
Heavy Atom Count	27
Complexity	522
Defined Atom Stereocenter Count	0
InChi Key	KOHMUAMPZUHGNV-UHFFFAOYSA-N
InChi Code	InChI=1S/C22H25ClO4/c1-13-14(2)19(7-5-15(13)6-8-20(24)25)26-12-17-10-18(23)9-16-11-22(3,4)27-21(16)17/h5,7,9-10H,6,8,11-12H2,1-4H3,(H,24,25)
Chemical Name	3-[4-[(5-chloro-2,2-dimethyl-3H-1-benzofuran-7-yl)methoxy]-2,3-dimethylphenyl]propanoic acid
Synonyms	GPR120 Agonist 2; MDK4111; MDK-4111; MDK 4111
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	GPR120
ln Vitro	In vitro activity: GPR120 Agonist 2, a GPR120 agonist, is helpful in treating metabolic disorders, such as Type II diabetes and conditions linked to inadequate glycemic control[1].
ln Vivo
Enzyme Assay	Treatment for metabolic disorders, such as Type II diabetes and conditions linked to inadequate glycemic control, can be achieved with GPR120 Agonist 2.
Animal Protocol
References	[1]. Gpr120 receptor agonists and uses thereof. US20110313003.

Solubility Data

Solubility (In Vitro)

DMSO: ≥ 50 mg/mL (~128.6 mM) Water: <1mg/mL Ethanol: <1mg/mL

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 3.5 mg/mL (9.00 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 35.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 3.5 mg/mL (9.00 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 35.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.5714 mL	12.8571 mL	25.7142 mL
	5 mM	0.5143 mL	2.5714 mL	5.1428 mL
	10 mM	0.2571 mL	1.2857 mL	2.5714 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.