G-Protein antagonist peptide TFA is a truncated substance P-related peptide that competes with receptors for G protein binding. G-Protein antagonist peptide TFA inhibits M2 muscarinic cholinergic receptor (M2 mAChR) activation of Gi or Go or β-adrenergic receptor activation of Gs in recombinant phospholipid vesicles.

Physicochemical Properties

Molecular Formula	C59H65F3N12O11S
Molecular Weight	1207.28
Related CAS #	G-Protein antagonist peptide;143675-79-0
Appearance	White to off-white solid powder
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. G protein antagonists. A novel hydrophobic peptide competes with receptor for G protein binding. J Biol Chem. 1992;267(23):16237-16243.

Solubility Data

Solubility (In Vitro)

DMSO :~100 mg/mL (~82.83 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (2.07 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	0.8283 mL	4.1415 mL	8.2831 mL
	5 mM	0.1657 mL	0.8283 mL	1.6566 mL
	10 mM	0.0828 mL	0.4142 mL	0.8283 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.