Foxy-5 TFA is a WNT5A agonist, a mimetic peptide of WNT5A, and a non-classical member of the Wnt family. Foxy-5 TFA triggers cytoplasmic free calcium signaling without affecting β-catenin activation and inhibits epithelial cancer cell migration and invasion. Foxy-5 TFA can effectively reduce the metastatic spread of prostate cancer cells with low WNT5A expression in an orthotopic mouse model.

Physicochemical Properties

Molecular Formula	C28H43F3N6O14S2
Molecular Weight	808.80
Related CAS #	Foxy-5;881188-51-8
Appearance	White to off-white solid powder
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	WNT5A[1]
ln Vivo	In an orthotopic mouse model, Foxy-5 efficiently inhibits the metastatic spread of WNT5A-low prostate cancer cells[1]. In a xenograft mouse model of human colon cancer, Foxy-5 decreases the quantity of colonic cancer stem cells[2].
References	[1]. Säfholm A, et al. The Wnt-5a-derived hexapeptide Foxy-5 inhibits breast cancer metastasis in vivo by targeting cell motility. Clin Cancer Res. 2008 Oct 15;14(20):6556-63. [2]. Osman J, et al. The WNT5A Agonist Foxy5 Reduces the Number of Colonic Cancer Stem Cells in a Xenograft Mouse Model of Human Colonic Cancer. Anticancer Res. 2019 Apr;39(4):1719-1728. [3]. Canesin G, et al. Treatment with the WNT5A-mimicking peptide Foxy-5 effectively reduces the metastatic spread of WNT5A-low prostate cancer cells in an orthotopic mouse model. PLoS One. 2017 Sep 8;12(9):e0184418. [4]. Kelsey R. Prostate cancer: Foxy-5 in prostate cancer model. Nat Rev Urol. 2017 Nov;14(11):638.

Solubility Data

Solubility (In Vitro)	H2O :~20 mg/mL (~24.73 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 7.14 mg/mL (8.83 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.2364 mL	6.1820 mL	12.3640 mL
	5 mM	0.2473 mL	1.2364 mL	2.4728 mL
	10 mM	0.1236 mL	0.6182 mL	1.2364 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.