FAK-IN-22 (Compound 26) is an inhibitor of FAK, JAK3, and Aurora B with IC50 values of 50.94 nM, 9.99 nM, and 0.49 nM, respectively, and effectively inhibits the tumorigenesis and metastasis of pancreatic ductal adenocarcinoma (PDAC). FAK-IN-22 effectively inhibits the proliferation of PANC-1 cells with an IC50 value of 0.15 μM. FAK-IN-22 induces apoptosis and G2/M arrest in PANC-1 cells by inhibiting the FAK/PI3K/Akt signaling pathway.

Physicochemical Properties

Molecular Formula	C21H16F3N5O2
Molecular Weight	427.38
CAS #	2703920-02-7
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	JAK3 9.99 nM (IC50); FAK 50.94 nM (IC50); Aurora B 0.49 nM (IC50)
References	[1]. Discovery of a potent anticancer agent against pancreatic ductal adenocarcinoma targeting FAK with DFG-out state and JAK/Aurora kinases. Eur J Med Chem. 2025 Jan 15:282:117059.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.3398 mL	11.6992 mL	23.3984 mL
	5 mM	0.4680 mL	2.3398 mL	4.6797 mL
	10 mM	0.2340 mL	1.1699 mL	2.3398 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.