Erythrosine sodium (close form, CI-45430 CI45430; CI 45430) is a tetraiodofluorescein and a dye used as a fluorescent indicator in microscopy and for adsorption.

Physicochemical Properties

Molecular Formula	C20H6I4NA2O5
Molecular Weight	879.86
Exact Mass	879.618
CAS #	16423-68-0
Related CAS #	15905-32-5 (parent cpd)
PubChem CID	12961638
Appearance	Pink to red solid powder
Density	0.98
Boiling Point	693.2ºC at 760 mmHg
Melting Point	>300ºC (Decomposes)
Flash Point	373ºC
Vapour Pressure	2.22E-16mmHg at 25°C
LogP	6.96
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	0
Heavy Atom Count	31
Complexity	645
Defined Atom Stereocenter Count	0
InChi Key	RAGZEDHHTPQLAI-UHFFFAOYSA-L
InChi Code	InChI=1S/C20H8I4O5.2Na/c21-11-5-9-17(13(23)15(11)25)28-18-10(6-12(22)16(26)14(18)24)20(9)8-4-2-1-3-7(8)19(27)29-20;;/h1-6,25-26H;;/q;2*+1/p-2
Chemical Name	disodium;2',4',5',7'-tetraiodo-3-oxospiro[2-benzofuran-1,9'-xanthene]-3',6'-diolate
Synonyms	CI-45430 CI45430 CI 45430 Erythrosine sodium (close form)
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	The only concentrations of erythrosine B that differed substantially from the carrier cosmetic (p<0.05) were the two highest tested concentrations (50.0 and 70.0 μg/mL), when measuring tail shock and tail intensity, which indicate the degree of DNA damage. In comparison to the vehicle control group, the frequency of six micronuclei (MNi) increased across seven erythrosine B concentrations (0.2 to 70.0 μg/mL) [1].
References	[1]. Establishing an experimental rat model of photodynamically-induced retinal vein occlusion using erythrosin B. Int J Ophthalmol. 2014 Apr 18;7(2):232-8. [2]. Genotoxic and mutagenic effects of erythrosine B, a xanthene food dye, on HepG2 cells. Food Chem Toxicol. 2012 Oct;50(10):3447-51.
Additional Infomation	A tetraiodofluorescein used as a red coloring in some foods (cherries, fish), as a disclosure of DENTAL PLAQUE, and as a stain of some cell types. It has structural similarity to THYROXINE.

Solubility Data

Solubility (In Vitro)

H2O : ~33.33 mg/mL (~37.88 mM) DMSO : ~33.33 mg/mL (~37.88 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (2.84 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (2.84 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: 33.33 mg/mL (37.88 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.1365 mL	5.6827 mL	11.3654 mL
	5 mM	0.2273 mL	1.1365 mL	2.2731 mL
	10 mM	0.1137 mL	0.5683 mL	1.1365 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.