Physicochemical Properties
| Molecular Formula | C7H7N3 |
| Molecular Weight | 133.150580644608 |
| CAS # | 8001-17-0 |
| Appearance | Liquid |
| SMILES | N1=C2C=CC=CC2=NC1N |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| ln Vitro | Egg oil (0.1-0.8 mg/ml) can reduce IL-8 secretion and exert anti-inflammatory activity through the Nrf2/NF-kB signaling pathway[2]. Egg oil (10-600 μg/ml) (extracted from sea urchin) attenuates 7β-OHC-induced cytotoxicity, regulates the activity of antioxidant enzymes and reduces the production of MDA and CD, thereby showing a neuroprotective effect in alleviating neurodegenerative diseases[4]. |
| ln Vivo | Egg oil (150-600 mg/kg, gavage, 16 weeks) (extracted from the swimming crab) can improve insulin sensitivity and liver glycogen synthesis, and alleviate insulin resistance by activating the PI3K/Akt/Glu4 signaling pathway in skeletal muscle and the PI3K/Akt/GSK3β/GS signaling pathway in the liver[1]. Egg oil (150-600 mg/kg, gavage, 16 weeks) (extracted from the swimming crab) can improve HFD-induced obesity in mice, restore intestinal microbial flora imbalance and downregulate the LPS/TLR4 pathway in liver and epididymal fat[3]. |
| Cell Assay |
Cell Viability Assay[2] Cell Types: Caco2 Concentration: 0.1-0.8 mg/ml Incubation Duration: 2 h Experimental Results: Maintained cell viability. |
| Animal Protocol |
Animal/Disease Models:High-fat-diet feeding C57BL/6J mice[1][3] Doses: 150-600 mg/kg Route of Administration: i.g. for 16 weeks Experimental Results: Reduced body weight, adipose weight gain, blood glucose and lipids. Increased mRNA levels of PI3K, Akt, IPS1/2, Glu4, GSK3β and GS i muscle and liver. |
| References |
[1]. Egg oil from Portunus trituberculatus alleviates insulin resistance through activation of insulin signaling in mice. Appl Physiol Nutr Metab. 2019 Oct;44(10):1081-1088. [2]. , Egg yolk oils exert anti-inflammatory effect via regulating Nrf2/NF-κB pathway. J Ethnopharmacol. 2021 Jun 28;274:114070. [3]. , Egg oil from Portunus trituberculatus alleviated obesity and regulated gut microbiota in mice. Sci Rep. 2020 May 21;10(1):8454. [4]. , 7β-hydroxycholesterol-induced cell death, oxidative stress, and fatty acid metabolism dysfunctions attenuated with sea urchin egg oil. Biochimie. 2018 Oct;153:210-219. |
Solubility Data
| Solubility (In Vitro) | May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples |
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples. Injection Formulations (e.g. IP/IV/IM/SC) Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] *Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline) Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline) Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline) Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil) Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Oral Formulations Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). Oral Formulation 3: Dissolved in PEG400 Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose Oral Formulation 6: Mixing with food powders Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 7.5103 mL | 37.5516 mL | 75.1033 mL | |
| 5 mM | 1.5021 mL | 7.5103 mL | 15.0207 mL | |
| 10 mM | 0.7510 mL | 3.7552 mL | 7.5103 mL |