EGFR kinase inhibitor 6 (Compound 4b) is an orally available epidermal growth factor receptor (EGFR) kinase inhibitor with IC50 of 24.34 μM. EGFR kinase inhibitor 6 induces cell apoptosis. EGFR kinase inhibitor 6 has anticancer and anti-inflammatory activities, and low toxicity (LD50 ranges from 500-2000 mg/kg).

Physicochemical Properties

Molecular Formula	C21H16CLN3OS2
Molecular Weight	425.95
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	IC50: 24.34 μM (EGFR Kinase)
ln Vitro	EGFR kinase inhibitor 5 (0-80 μM, 72 h) inhibits the migration of cancer cells A549 and the activity of cancer cells A549, MCF7 and HCT116, with IC50 values of 21.06, 29.25 and >60 μM, respectively[1]. Cell Migration Assay [1] Cell Line: A549 Concentration: 0-30 μM Incubation Time: 24 h Result: Inhibited cell migration.
ln Vivo	EGFR kinase inhibitor 6 (13.37 mg/kg, oral, 15 days) exhibited anti-inflammatory activity in the carrageenan-induced rat paw edema model and was less likely to induce ulceration and lipid peroxidation [1].
Animal Protocol	Animal/Disease Models: carrageenan-induced hind paw edema in Wistar rats model[1] Doses: 0.0314 mmol/kg Route of Administration: p.o., 15 days Experimental Results: Inhibited 81.34% paw edema.
References	[1]. Design, synthesis, biological assessment and molecular modeling studies of novel imidazothiazole-thiazolidinone hybrids as potential anticancer and anti-inflammatory agents. Sci Rep. 2024 Apr 11;14(1):8457.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.3477 mL	11.7385 mL	23.4769 mL
	5 mM	0.4695 mL	2.3477 mL	4.6954 mL
	10 mM	0.2348 mL	1.1738 mL	2.3477 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.