(E)-2-Hexadecenal (trans-2-Hexadecenal) is a MLK3 activator that induces cytoskeletal reorganization in human and mouse cells, leading to cell rounding, detachment, and ultimately apoptosis. (E)-2-Hexadecenal activates the MLK3 signaling pathway, phosphorylates MKK4/7 and JNK, and then activates JNK downstream targets such as c-Jun phosphorylation, cytochrome c release, Bax activation, Bid cleavage, and Bim translocation to mitochondria. (E)-2-Hexadecenal has application value in the field of sphingolipid signaling pathway research.

Physicochemical Properties

Molecular Formula	C16H30O
Molecular Weight	238.41
CAS #	22644-96-8
SMILES	CCCCCCCCCCCCC/C=C/C=O
Synonyms	trans-2-Hexadecenal
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. The sphingolipid degradation product trans-2-hexadecenal induces cytoskeletal reorganization and apoptosis in a JNK-dependent manner. Cell Signal. 2011 Jul;23(7):1144-52.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.1945 mL	20.9723 mL	41.9445 mL
	5 mM	0.8389 mL	4.1945 mL	8.3889 mL
	10 mM	0.4194 mL	2.0972 mL	4.1945 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.