Dimethyl-bisphenol A (DMBPA) is a potent HIF-1α inhibitor. Dimethyl-bisphenol A reduces Vegfa mRNA expression.

Physicochemical Properties

Molecular Formula	C17H20O2
Molecular Weight	256.34
Exact Mass	256.146
CAS #	1568-83-8
PubChem CID	617780
Appearance	White to off-white solid powder
Density	1.022g/cm3
Boiling Point	364.8ºC at 760 mmHg
Melting Point	54-59ºC
Flash Point	136.3ºC
Vapour Pressure	3.45E-05mmHg at 25°C
Index of Refraction	1.532
LogP	4.029
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	4
Heavy Atom Count	19
Complexity	232
Defined Atom Stereocenter Count	0
InChi Key	OJYIBEYSBXIQOP-UHFFFAOYSA-N
InChi Code	InChI=1S/C17H20O2/c1-17(2,13-5-9-15(18-3)10-6-13)14-7-11-16(19-4)12-8-14/h5-12H,1-4H3
Chemical Name	1-methoxy-4-[2-(4-methoxyphenyl)propan-2-yl]benzene
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	In big osteoclasts (OC), the expression of Vegfa120 and Vegfa164 is strongly inhibited by dimethylbisphenol A (DMBPA; 50 μM; 30 minutes) [1].
References	[1]. VEGF-A expression in osteoclasts is regulated by NF-kappaB induction of HIF-1alpha. J Cell Biochem. 2010 May 15;110(2):343-51.

Solubility Data

Solubility (In Vitro)

DMSO : ≥ 100 mg/mL (~390.11 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (9.75 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.75 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.9011 mL	19.5053 mL	39.0107 mL
	5 mM	0.7802 mL	3.9011 mL	7.8021 mL
	10 mM	0.3901 mL	1.9505 mL	3.9011 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.