Dibenamine HCl is a competitive and irreversible adrenergic blocker that can modify the pharmacological effects of epinephrine. Dibenamine HCl significantly increased the destruction of epinephrine in mice.

Physicochemical Properties

Molecular Formula	C16H19CL2N
Molecular Weight	296.23
Exact Mass	295.089
CAS #	55-43-6
PubChem CID	5925
Appearance	White to light yellow solid powder
Density	1.107g/cm3
Boiling Point	320ºC at 760mmHg
Melting Point	190-193 °C(lit.)
Flash Point	147.3ºC
LogP	4.729
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	1
Rotatable Bond Count	6
Heavy Atom Count	19
Complexity	187
Defined Atom Stereocenter Count	0
SMILES	C1=CC=C(C=C1)CN(CCCl)CC2=CC=CC=C2.Cl
InChi Key	LZXCEBPGNFLHEQ-UHFFFAOYSA-N
InChi Code	InChI=1S/C16H18ClN.ClH/c17-11-12-18(13-15-7-3-1-4-8-15)14-16-9-5-2-6-10-16;/h1-10H,11-14H2;1H
Chemical Name	N,N-dibenzyl-2-chloroethanamine;hydrochloride
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	IC50: Adrenergic receptor[1]
ln Vitro	In the isolated vas deferens of guinea pigs, dibenamine (100 nM–10 μM) attenuates the degree of cocaine-induced increase in acetylcholine sensitivity while having no effect on cholinergic contraction. Furthermore, the degree of dibenamine-induced inhibition is negatively correlated with cocaine concentration and depends on dibenamine concentration[3].
ln Vivo	Subcutaneous injection of 25 mg/kg dibenamine hydrochloride 48 and 24 hours prior to CCl4 administration reduces CHCl3 levels by 30–50% at 2 and 6 hours, but has no discernible effect on the half-life of CHCl3 in the liver. The conversion of Ccl4 to CHCL3 appears to be slowed down by pretreatment with dibenzamine[1].
References	[1]. R W SCHAYER, et al. Effect of dibenamine (N-(2-chloroethyl) dibenzylamine) on the metabolism of radioactive epinephrine.J Biol Chem. 1953 May;202(1):39-43. [2]. H M Maling,et al. Nature of the protection against carbon tetrachloride-induced hepatotoxicity produced by pretreatment with dibenamine (N-(2-chloroethyl)dibenzylamine). Biochem Pharmacol. 1974 May 15;23(10):1479-91. [3]. K Araki, et al. Pharmacological studies on supersensitization. VII. Inhibitory effect of dibenamine on cocaine-induced supersensitivity of isolated vas deferens of guinea pig. J Pharmacobiodyn. 1982 Oct;5(10):789-95.

Solubility Data

Solubility (In Vitro)

DMSO: 125 mg/mL (421.97 mM) H2O: 33.33 mg/mL (112.51 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (7.02 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (7.02 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.08 mg/mL (7.02 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. Solubility in Formulation 4: 9.09 mg/mL (30.69 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication (<60°C).  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.3758 mL	16.8788 mL	33.7576 mL
	5 mM	0.6752 mL	3.3758 mL	6.7515 mL
	10 mM	0.3376 mL	1.6879 mL	3.3758 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.