DiZPK is novel photocrosslinking agent, revealing chaperone cooperation in acid resistance. DiZPK is photocrosslinking amino acid, could be significant in revealling the physiological and functions of some proteins acting as a genetically encoded.

Physicochemical Properties

Molecular Formula	C12H23N5O3
Molecular Weight	285.34
Exact Mass	285.18
CAS #	1337883-32-5
Related CAS #	DiZPK hydrochloride;2349295-23-2
PubChem CID	53314876
Appearance	White to off-white solid powder
LogP	1.183
Hydrogen Bond Donor Count	4
Hydrogen Bond Acceptor Count	6
Rotatable Bond Count	10
Heavy Atom Count	20
Complexity	366
Defined Atom Stereocenter Count	1
SMILES	CC1(N=N1)CCCNC(=O)NCCCC[C@@H](C(=O)O)N
InChi Key	YGUIFRMUMYTGAV-VIFPVBQESA-N
InChi Code	InChI=1S/C12H23N5O3/c1-12(16-17-12)6-4-8-15-11(20)14-7-3-2-5-9(13)10(18)19/h9H,2-8,13H2,1H3,(H,18,19)(H2,14,15,20)/t9-/m0/s1
Chemical Name	N6-((3-(3-Methyl-3H-diazirin-3-yl)propyl)carbamoyl)-L-lysine
Synonyms	DiZPK CS-4117 EBP 883 BMS 790052
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	In order to detect direct protein-protein interactions in living prokaryotic and eukaryotic cells, DiZPK, a structural analog of pyrrolysine (Pyl), functions as a photocross-linker [1].
References	[1]. A genetically incorporated crosslinker reveals chaperone cooperation in acid resistance. Nat Chem Biol. 2011 Sep 4;7(10):671-7.

Solubility Data

Solubility (In Vitro)	H2O : ~5.2 mg/mL (~18.22 mM) DMSO : ~5 mg/mL (~17.52 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 2 mg/mL (7.01 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.5046 mL	17.5230 mL	35.0459 mL
	5 mM	0.7009 mL	3.5046 mL	7.0092 mL
	10 mM	0.3505 mL	1.7523 mL	3.5046 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.