DRP1i27 is a potent inhibitor of human Drp1 (dynamin-related protein 1). DRP1i27 binds to the GTPase site of Drp1 and forms hydrogen bonds with Gln34 and Asp218. DRP1i27 targets Drp1-mediated mitochondrial fission and prevents simulated ischemia-reperfusion injury in cell line models.

Physicochemical Properties

Molecular Formula	C20H26N6O
Molecular Weight	366.460043430328
Exact Mass	366.216
CAS #	1453028-33-5
Related CAS #	DRP1i27 dihydrochloride
PubChem CID	72901225
Appearance	Light yellow to brown solid powder
LogP	1.5
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	5
Heavy Atom Count	27
Complexity	507
Defined Atom Stereocenter Count	2
SMILES	[C@]12([H])CC[C@]([H])(N(CC3=CC=C(C4C=CNN=4)O3)C1)CN(CC1NC=NC=1C)C2
InChi Key	PJYHAZFCIWUKAT-JKSUJKDBSA-N
InChi Code	InChI=1S/C20H26N6O/c1-14-19(22-13-21-14)12-25-8-15-2-3-16(10-25)26(9-15)11-17-4-5-20(27-17)18-6-7-23-24-18/h4-7,13,15-16H,2-3,8-12H2,1H3,(H,21,22)(H,23,24)/t15-,16+/m0/s1
Chemical Name	(1S,5R)-3-[(5-methyl-1H-imidazol-4-yl)methyl]-6-[[5-(1H-pyrazol-5-yl)furan-2-yl]methyl]-3,6-diazabicyclo[3.2.2]nonane
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Human Drp1's GTPase activity is directly bound to and inhibited by DRP1i27 (0-50 µM) [1]. In human and animal fibroblasts, DRP1i27 (0-50 µM) enhances the cellular network of mitochondria in a Drp1-dependent manner [1]. In the SPR assay, DRP1i27 has a binding affinity of 286 µM, while in the MST assay, its KD value is 190 µM [1].
References	[1]. A novel small molecule inhibitor of human Drp1. Sci Rep. 2022 Dec 13;12(1):21531.

Solubility Data

Solubility (In Vitro)

DMSO : 100 mg/mL (272.88 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (6.82 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.82 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.7288 mL	13.6441 mL	27.2881 mL
	5 mM	0.5458 mL	2.7288 mL	5.4576 mL
	10 mM	0.2729 mL	1.3644 mL	2.7288 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.