DL-Norepinephrine HCl is a synthetic phenylethylamine that mimics the sympathomimetic effects of endogenous norepinephrine. DL-Norepinephrine HCl is a neurotransmitter that targets α1 adrenoceptor and β1 adrenoceptor and reduces subendocardial oxygen tension.

Physicochemical Properties

Molecular Formula	C8H12CLNO3
Molecular Weight	205.64
Exact Mass	205.05
CAS #	55-27-6
Related CAS #	DL-Norepinephrine-d6 hydrochloride;1219803-04-9;DL-Norepinephrine-d3 hydrochloride;1392208-07-9
PubChem CID	5923
Appearance	White to light brown solid powder
Density	1.397g/cm3
Boiling Point	442.6ºC at 760mmHg
Melting Point	140-144ºC (dec.)
Flash Point	221.5ºC
LogP	1.592
Hydrogen Bond Donor Count	5
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	2
Heavy Atom Count	13
Complexity	142
Defined Atom Stereocenter Count	0
InChi Key	FQTFHMSZCSUVEU-UHFFFAOYSA-N
InChi Code	InChI=1S/C8H11NO3.ClH/c9-4-8(12)5-1-2-6(10)7(11)3-5;/h1-3,8,10-12H,4,9H2;1H
Chemical Name	4-(2-amino-1-hydroxyethyl)benzene-1,2-diol;hydrochloride
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	IC50: α1 adrenoceptor and β1 adrenoceptor[1]
References	[1]. Effects of nitroglycerin, adenosine, noradrenaline, and isoproterenol on the myocardial oxygen tension. Jpn Heart J. 1979 Nov;20(6):867-79.
Additional Infomation	Precursor of epinephrine that is secreted by the ADRENAL MEDULLA and is a widespread central and autonomic neurotransmitter. Norepinephrine is the principal transmitter of most postganglionic sympathetic fibers, and of the diffuse projection system in the brain that arises from the LOCUS CERULEUS. It is also found in plants and is used pharmacologically as a sympathomimetic.

Solubility Data

Solubility (In Vitro)

1M HCl: 100 mg/mL (486.29 mM) DMSO: 50 mg/mL (243.14 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 0.89 mg/mL (4.33 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 8.9 mg/mL clear DMSO stock solution to 400 μL of PEG300 and mix evenly; then add 50 μL of Tween-80 to the above solution and mix evenly; then add 450 μL of normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 0.89 mg/mL (4.33 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 8.9 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 0.89 mg/mL (4.33 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 8.9 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.8629 mL	24.3143 mL	48.6287 mL
	5 mM	0.9726 mL	4.8629 mL	9.7257 mL
	10 mM	0.4863 mL	2.4314 mL	4.8629 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.