DHBS is used together with 4-aminoantipyrine (4-AAP) and hydrogen peroxide (H2O2) for chromogenic quantification of peroxidase in coupled enzymatic reactions. A component of Trinder's reagent used with peroxidase to measure hydrogen peroxide production.

Physicochemical Properties

Molecular Formula	C6H3CL2NAO4S
Molecular Weight	265.0464
Exact Mass	263.902
CAS #	54970-72-8
Related CAS #	26281-43-6 (Parent)
PubChem CID	3085077
Appearance	White to off-white solid powder
Melting Point	>300°C
LogP	2.683
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	1
Heavy Atom Count	14
Complexity	278
Defined Atom Stereocenter Count	0
InChi Key	NMWCVZCSJHJYFW-UHFFFAOYSA-M
InChi Code	InChI=1S/C6H4Cl2O4S.Na/c7-3-1-4(8)6(9)5(2-3)13(10,11)12;/h1-2,9H,(H,10,11,12);/q;+1/p-1
Chemical Name	sodium;3,5-dichloro-2-hydroxybenzenesulfonate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. In vitro oxidation of hispidin and gallic acid by horseradish peroxidase. J Biomol Struct Dyn. 2023 Apr;41(6):2321-2325. [2]. Aizenman E. Modulation of N-methyl-D-aspartate receptors by hydroxyl radicals in rat cortical neurons in vitro. Neurosci Lett. 1995 Apr 7;189(1):57-9. [3]. The analysis of serum urate utilizing immobilized uricase. Appl Biochem Biotechnol. 1981 Mar;6(1):15-24.

Solubility Data

Solubility (In Vitro)	H2O : ~100 mg/mL (~377.29 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 25 mg/mL (94.32 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.7729 mL	18.8644 mL	37.7287 mL
	5 mM	0.7546 mL	3.7729 mL	7.5457 mL
	10 mM	0.3773 mL	1.8864 mL	3.7729 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.