DBCO-Maleimide is a cleavable (degradable) ADC (Antibody-drug conjugate) linker used for the synthesis of antibody-conjugated active molecules (ADCs). DBCO-Maleimide is a reagent for click chemistry. It has a DBCO group and could undergo SPAAC (Strain-promoted alkyne-azide cycloaddition) with compounds bearing an Azide (N3) moiety.

Physicochemical Properties

Molecular Formula	C25H21N3O4
Molecular Weight	427.4519
Exact Mass	427.153
CAS #	1395786-30-7
PubChem CID	77078149
Appearance	Light yellow to brown solid powder
Density	1.4±0.1 g/cm3
Boiling Point	790.5±60.0 °C at 760 mmHg
Flash Point	431.9±32.9 °C
Vapour Pressure	0.0±2.8 mmHg at 25°C
Index of Refraction	1.688
LogP	3.1
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	6
Heavy Atom Count	32
Complexity	849
Defined Atom Stereocenter Count	0
InChi Key	NHFQNAGPXIVKND-UHFFFAOYSA-N
InChi Code	InChI=1S/C25H21N3O4/c29-22(14-16-27-23(30)11-12-24(27)31)26-15-13-25(32)28-17-20-7-2-1-5-18(20)9-10-19-6-3-4-8-21(19)28/h1-8,11-12H,13-17H2,(H,26,29)
Chemical Name	N-[3-(2-azatricyclo[10.4.0.04,9]hexadeca-1(16),4,6,8,12,14-hexaen-10-yn-2-yl)-3-oxopropyl]-3-(2,5-dioxopyrrol-1-yl)propanamide
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	The ADC is made up of a preamplifier, to which the ADC input connects the ADC cytotoxic.
References	[1]. Modified antibody and radioactive metal-labelled antibody. WO2019203191A1.

Solubility Data

Solubility (In Vitro)

DMSO : ~125 mg/mL (~292.43 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (4.87 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (4.87 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.08 mg/mL (4.87 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.3395 mL	11.6973 mL	23.3945 mL
	5 mM	0.4679 mL	2.3395 mL	4.6789 mL
	10 mM	0.2339 mL	1.1697 mL	2.3395 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.