Physicochemical Properties
| Molecular Formula | C16H17CL2N5 |
| Molecular Weight | 350.24 |
| Exact Mass | 349.086 |
| Elemental Analysis | C, 54.87; H, 4.89; Cl, 20.24; N, 20.00 |
| CAS # | 28718-90-3 |
| Related CAS # | 47165-04-8 |
| PubChem CID | 160166 |
| Appearance | Light yellow to green yellow solid powder |
| Density | 1.41g/cm3 |
| Boiling Point | 545.4ºC at 760mmHg |
| Melting Point | >300°C (dec.) |
| Flash Point | 283.6ºC |
| Vapour Pressure | 5.96E-12mmHg at 25°C |
| LogP | 5.607 |
| Hydrogen Bond Donor Count | 7 |
| Hydrogen Bond Acceptor Count | 2 |
| Rotatable Bond Count | 3 |
| Heavy Atom Count | 23 |
| Complexity | 413 |
| Defined Atom Stereocenter Count | 0 |
| SMILES | Cl[H].Cl[H].N1([H])C(C2C([H])=C([H])C(/C(=N/[H])/N([H])[H])=C([H])C=2[H])=C([H])C2C([H])=C([H])C(/C(=N/[H])/N([H])[H])=C([H])C1=2 |
| InChi Key | FPNZBYLXNYPRLR-UHFFFAOYSA-N |
| InChi Code | InChI=1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H |
| Chemical Name | 2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide;dihydrochloride |
| Synonyms | FxCycle Violet; DAPI Dihydrochloride |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| ln Vitro | DAPI binds to the double-stranded DNA's minor groove, preferring to attach to adenine-thymine clusters. Permeabilization and/or fixation of cells are necessary for DAPI to penetrate and bind DNA. Approximately a 20-fold increase in fluorescence is observed upon DAPI binding to double-stranded DNA. [1] |
| References |
[1]. Biotech Histochem . 1995 Sep;70(5):220-33. |
Solubility Data
| Solubility (In Vitro) |
DMSO: 70~75 mg/mL (199.9~214.1 mM) H2O: ~5.6 mg/mL (~15.9 mM) |
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (5.94 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.94 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.08 mg/mL (5.94 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8552 mL | 14.2759 mL | 28.5519 mL | |
| 5 mM | 0.5710 mL | 2.8552 mL | 5.7104 mL | |
| 10 mM | 0.2855 mL | 1.4276 mL | 2.8552 mL |