Crustacean cardioactive peptide, free acid is a highly conserved nonapeptide, which was first extracted from the pericardial organ of the common shore crab. It has the function of regulating heartbeat; Crustacean cardioactive peptide, free acid can also regulate the heartbeat in other arthropods. neural activity.

Physicochemical Properties

Molecular Formula	C42H58N10O12S2
Molecular Weight	959.10
Exact Mass	958.367
CAS #	309247-84-5
PubChem CID	166638345
Appearance	White to off-white solid powder
LogP	-3.9
Hydrogen Bond Donor Count	14
Hydrogen Bond Acceptor Count	15
Rotatable Bond Count	26
Heavy Atom Count	66
Complexity	1720
Defined Atom Stereocenter Count	9
SMILES	C1C(C[C@H](NC([C@@H](NC(=O)[C@H](CC(N)=O)NC([C@H](CS)NC(=O)[C@H](CC2=CC=CC=C2)NC(=O)[C@@H]2CCCN2)=O)C)=O)C(N[C@H](C(NCC(N[C@@H](CS)C(O)=O)=O)=O)[C@@H](C)O)=O)=CC=CC=1
InChi Key	ONSAAPXCOZQERN-HNGYRCOKSA-N
InChi Code	InChI=1S/C42H58N10O12S2/c1-22(35(56)48-28(17-25-12-7-4-8-13-25)39(60)52-34(23(2)53)41(62)45-19-33(55)47-31(21-66)42(63)64)46-37(58)29(18-32(43)54)50-40(61)30(20-65)51-38(59)27(16-24-10-5-3-6-11-24)49-36(57)26-14-9-15-44-26/h3-8,10-13,22-23,26-31,34,44,53,65-66H,9,14-21H2,1-2H3,(H2,43,54)(H,45,62)(H,46,58)(H,47,55)(H,48,56)(H,49,57)(H,50,61)(H,51,59)(H,52,60)(H,63,64)/t22-,23+,26-,27-,28-,29-,30-,31-,34-/m0/s1
Chemical Name	(2R)-2-[[2-[[(2S,3R)-2-[[(2S)-2-[[(2S)-2-[[(2S)-4-amino-4-oxo-2-[[(2R)-2-[[(2S)-3-phenyl-2-[[(2S)-pyrrolidine-2-carbonyl]amino]propanoyl]amino]-3-sulfanylpropanoyl]amino]butanoyl]amino]propanoyl]amino]-3-phenylpropanoyl]amino]-3-hydroxybutanoyl]amino]acetyl]amino]-3-sulfanylpropanoic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	While abdominal-A and ultrabithorax are not necessary for the specification of CCAP interneurons, they are necessary to stop CCAP efferent neurons from dying from apoptosis [2].
ln Vivo	The mosquito auxiliary pulsatile organ (APO), heart contraction rate, and hemolymph flow rate in the antennal region are all increased by CCAP, FMRFamide, and SALDKNFMRFamide [1].
References	[1]. CCAP and FMRFamide-like peptides accelerate the contraction rate of the antennal accessory pulsatile organs (auxiliary hearts) of mosquitoes. J Exp Biol. 2016 Aug 1;219(Pt 15):2388-95. [2]. The study of the Bithorax-complex genes in patterning CCAP neurons reveals a temporal control of neuronal differentiation by Abd-B. Biol Open. 2015 Aug 14;4(9):1132-42.

Solubility Data

Solubility (In Vitro)

H2O : ~0.67 mg/mL (~0.70 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (2.61 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (2.61 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (2.61 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.0426 mL	5.2132 mL	10.4264 mL
	5 mM	0.2085 mL	1.0426 mL	2.0853 mL
	10 mM	0.1043 mL	0.5213 mL	1.0426 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.