Crocetin (Transcrocetin) meglumine, found in saffron (Crocus sativus L.), is a high-affinity NMDA receptor antagonist.

Physicochemical Properties

Molecular Formula	C34H58N2O14
Molecular Weight	718.83
Related CAS #	Crocetin disodium;591230-99-8;Crocetin;27876-94-4
Appearance	Typically exists as solid at room temperature
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	NMDA Receptor
ln Vitro	The saffron metabolite crocetin (Transcrocetin) meglumine, which comes from the crocin apocarotenoids, has been demonstrated to have a significant affinity for NMDA receptors and is assumed to be the cause of saffron's central nervous system activity.After a 24-hour incubation period with the test compounds, the cellular mitochondrial dehydrogenase activity of Caco-2 cells is measured by the MTT assay to ensure that the viability of the cells remains unchanged throughout the transport experiments. The results show that neither hydroalcoholic saffron extract (SE, 0.5-1 mg/mL) nor crocin-1 (250-1000 µM) significantly alter the viability of the cells. Cell viability is not affected by crocetin meglumine at 10 µM doses, but it is dramatically reduced at higher concentrations (40–160 µM)[1].
References	[1]. Intestinal formation of trans-Crocetin from saffron extract (Crocus sativus L.) and in vitro permeation through intestinal and blood brain barrier. Phytomedicine. 2015 Jan 15;22(1):36-44.

Solubility Data

Solubility (In Vitro)

DMSO :~8.33 mg/mL (~11.59 mM )

Solubility (In Vivo)

Solubility in Formulation 1: 0.83 mg/mL (1.15 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 8.3 mg/mL clear DMSO stock solution to 400 μL of PEG300 and mix evenly; then add 50 μL of Tween-80 to the above solution and mix evenly; then add 450 μL of normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 0.83 mg/mL (1.15 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 8.3 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.3911 mL	6.9557 mL	13.9115 mL
	5 mM	0.2782 mL	1.3911 mL	2.7823 mL
	10 mM	0.1391 mL	0.6956 mL	1.3911 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.