Clorprenaline HCl (NSC-334693; NSC334693; NSC 334693), the hydrochloride salt of Clorprenaline, is a potent β2-receptor agonist used as a bronchodilator with a significant expansion of the bronchial effect.

Physicochemical Properties

Molecular Formula	C11H17CL2NO
Molecular Weight	250.16
Exact Mass	267.079
Elemental Analysis	C, 52.81; H, 6.85; Cl, 28.34; N, 5.60; O, 6.40
CAS #	6933-90-0
Related CAS #	3811-25-4; 6933-90-0 (hydrochloride)
PubChem CID	23360
Appearance	White to off-white solid powder
Boiling Point	329.7ºC at 760 mmHg
Melting Point	165-169ºC
Flash Point	153.2ºC
LogP	3.564
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	4
Heavy Atom Count	15
Complexity	163
Defined Atom Stereocenter Count	0
SMILES	ClC1=C([H])C([H])=C([H])C([H])=C1C([H])(C([H])([H])N([H])C([H])(C([H])([H])[H])C([H])([H])[H])O[H].Cl[H]
InChi Key	CPCPUCRSKRHVAH-UHFFFAOYSA-N
InChi Code	InChI=1S/C11H16ClNO.ClH/c1-8(2)13-7-11(14)9-5-3-4-6-10(9)12;/h3-6,8,11,13-14H,7H2,1-2H3;1H
Chemical Name	1-(2-chlorophenyl)-2-(propan-2-ylamino)ethanol;hydrochloride
Synonyms	NSC-334693; NSC334693; NSC 334693
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	β2-receptor
ln Vitro	In vitro activity: In a single chromatographic run, the linear ranges for cleprenaline are 8.0-1.6 ×103 ng/mL, and the detection limits are 3.0 ng/mL. [1] A bronchodilator with affinity-interaction or separation by CE with charged CD-CZE sulfobuthylether (SBE)-β-CD or β-CD-phosphate with resolution greater than 1.2 is cleprenaline. The ideal separation condition for cleprenaline is 60 mM HP-β-CD.[2] The three primary ingredients of a clorprenaline compound tablet are cleprenaline hydrochloride (a), bromhexine hydrochloride (b), and decloxizine hydrochloride (c).[3]
ln Vivo
Animal Protocol
References	[1]. J Pharm Biomed Anal . 2005 Jun 1;38(1):166-72. [2]. Chromatographia, 1999, 49(7-8), 141-144. [3]. Yao Xue Xue Bao . 1990;25(5):357-61.
Additional Infomation	Clorprenaline Hydrochloride is the hydrochloride salt form of clorprenaline, a beta-2 adrenergic agonist with bronchodilator activity. Clorprenaline selectively binds to and activates beta-2 adrenergic receptors in bronchiolar smooth muscle, thereby causing stimulation of adenyl cyclase, the enzyme that catalyzes the conversion of adenosine triphosphate (ATP) to cyclic-3',5'-adenosine monophosphate (cAMP). Increased intracellular cAMP levels cause relaxation of bronchial smooth muscle. This increases air flow and prevents bronchospasms and may eventually lead to an improvement of airway function.

Solubility Data

Solubility (In Vitro)

DMSO: 50~100 mg/mL (199.8~399.7 mM) Water: ~50 mg/mL (~199.8 mM) Ethanol: ~50 mg/mL (~199.8 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (9.99 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.99 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (9.99 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. Solubility in Formulation 4: 100 mg/mL (399.74 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.9974 mL	19.9872 mL	39.9744 mL
	5 mM	0.7995 mL	3.9974 mL	7.9949 mL
	10 mM	0.3997 mL	1.9987 mL	3.9974 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.