CXCR2 antagonist 8 is a potent and specific CXCR2 antagonist. CXCR2 antagonist 8 may be utilized in insulin resistance research.

Physicochemical Properties

Molecular Formula	C14H13N3O5
Molecular Weight	303.27
Exact Mass	303.085
CAS #	182498-30-2
PubChem CID	9796445
Appearance	Light yellow to brown solid powder
LogP	1.9
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	3
Heavy Atom Count	22
Complexity	400
Defined Atom Stereocenter Count	0
SMILES	OC1=CC([N+]([O-])=O)=CC=C1NC(NC1=CC=CC=C1OC)=O
InChi Key	KNPLCJZGNRGZAN-UHFFFAOYSA-N
InChi Code	InChI=1S/C14H13N3O5/c1-22-13-5-3-2-4-11(13)16-14(19)15-10-7-6-9(17(20)21)8-12(10)18/h2-8,18H,1H3,(H2,15,16,19)
Chemical Name	1-(2-hydroxy-4-nitrophenyl)-3-(2-methoxyphenyl)urea
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Cxcr2 receptor antagonists for the treatment or the prevention of insulin resistance. WO2010092440A1.

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~329.74 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (8.24 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.24 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.2974 mL	16.4870 mL	32.9739 mL
	5 mM	0.6595 mL	3.2974 mL	6.5948 mL
	10 mM	0.3297 mL	1.6487 mL	3.2974 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.