COQ7-IN-2 (compound 12) is an inhibitor (blocker/antagonist) of COQ7 with IC50s of 7.3 μM and 15.4 μM for DMQ10 and UQ10 accumulation, respectively.

Physicochemical Properties

Molecular Formula	C15H13N3O
Molecular Weight	251.2832
Exact Mass	251.105
CAS #	2579696-76-5
PubChem CID	155981981
Appearance	White to off-white solid powder
LogP	2.7
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	2
Heavy Atom Count	19
Complexity	374
Defined Atom Stereocenter Count	0
InChi Key	SKJOGGJAXXEJKE-UHFFFAOYSA-N
InChi Code	InChI=1S/C15H13N3O/c1-11-7-8-14(16-10-11)18-15(19)9-13(17-18)12-5-3-2-4-6-12/h2-10,17H,1H3
Chemical Name	2-(5-methylpyridin-2-yl)-5-phenyl-1H-pyrazol-3-one
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	COQ7-IN-2 (compound 12) exhibits lower growth inhibition when it comes to human normal culture cells[1]. Both PANC-1 cells (8.7% by COQ7-IN-2 (compound 12)) and PC3 cells (14.0 % by COQ7-IN-2 (compound 12)) exhibited significant DMQ10 accumulation[1].
References	[1]. Keiko Tsuganezawa, et al. Identification of small molecule inhibitors of human COQ7. Bioorg Med Chem. 2020 Jan 1;28(1):115182.

Solubility Data

Solubility (In Vitro)

DMSO: 25 mg/mL (99.49 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (9.95 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.9796 mL	19.8981 mL	39.7962 mL
	5 mM	0.7959 mL	3.9796 mL	7.9592 mL
	10 mM	0.3980 mL	1.9898 mL	3.9796 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.