CL264 is a TLR7-specific agonist that may be utilized to study innate immune signaling pathways.

Physicochemical Properties

Molecular Formula	C₁₉H₂₃N₇O₄
Molecular Weight	413.43
Exact Mass	413.181
CAS #	1510712-69-2
PubChem CID	90362358
Appearance	Brown to gray solid powder
Density	1.427±0.06 g/cm3(Predicted)
LogP	0.9
Hydrogen Bond Donor Count	5
Hydrogen Bond Acceptor Count	8
Rotatable Bond Count	9
Heavy Atom Count	30
Complexity	626
Defined Atom Stereocenter Count	0
InChi Key	BXJWQQWEBUICHY-UHFFFAOYSA-N
InChi Code	InChI=1S/C19H23N7O4/c1-2-3-8-21-18-24-15(20)14-16(25-18)26(19(30)23-14)10-11-4-6-12(7-5-11)17(29)22-9-13(27)28/h4-7H,2-3,8-10H2,1H3,(H,22,29)(H,23,30)(H,27,28)(H3,20,21,24,25)
Chemical Name	2-(4-((6-amino-2-(butylamino)-8-hydroxy-9H_purin_9-yl)methyl)benzamido)acetic acid
Synonyms	CL264 CL-264 CL 264
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	The most thoroughly researched class of receptors are toll-like receptors (TLRs), which are crucial for identifying a variety of base-, protein-, and sequence-based PAMPs. The majority of plasmacytoid dendritic cells (pDC) express TLR7. TLR7 modulators include adjuvants for vaccines, interferon inducers, anticancer, anti-infectious, and immune replacements [1].
References	[1]. TLR9-Activating CpG-B ODN but Not TLR7 Agonists Triggers Antibody Formation to Factor IX in Muscle Gene Transfer. Hum Gene Ther Methods. 2019 Jun;30(3):81-92.

Solubility Data

Solubility (In Vitro)

DMSO : ~25 mg/mL (~60.47 mM) H2O : < 0.1 mg/mL

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (5.03 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.4188 mL	12.0939 mL	24.1879 mL
	5 mM	0.4838 mL	2.4188 mL	4.8376 mL
	10 mM	0.2419 mL	1.2094 mL	2.4188 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.