CC-17369 (7-Hydroxy pomalidomide) is a metabolite of Pomalidomide. CC-17369 Pomalidomide-based cereblon (CRBN) ligand. CC-17369 can be linked to protein ligands via linkers to prepare PROTACs.

Physicochemical Properties

Molecular Formula	C13H11N3O5
Molecular Weight	289.25
Exact Mass	289.069
CAS #	1547162-46-8
PubChem CID	86709849
Appearance	Light brown to brown solid powder
Density	1.7±0.1 g/cm3
Boiling Point	649.7±55.0 °C at 760 mmHg
Flash Point	346.7±31.5 °C
Vapour Pressure	0.0±2.0 mmHg at 25°C
Index of Refraction	1.725
LogP	-1.97
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	6
Rotatable Bond Count	1
Heavy Atom Count	21
Complexity	534
Defined Atom Stereocenter Count	0
InChi Key	DNODJHQYSZVNMH-UHFFFAOYSA-N
InChi Code	InChI=1S/C13H11N3O5/c14-5-1-3-7(17)10-9(5)12(20)16(13(10)21)6-2-4-8(18)15-11(6)19/h1,3,6,17H,2,4,14H2,(H,15,18,19)
Chemical Name	4-amino-2-(2,6-dioxopiperidin-3-yl)-7-hydroxyisoindole-1,3-dione
Synonyms	CC17369; CC 17369; CC-17369
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Absorption, metabolism and excretion of [14C]pomalidomide in humans following oral administration. Cancer Chemother Pharmacol. 2013;71(2):489-501. [2]. Slowly folding surface extension in the prototypic avian hepatitis B virus capsid governs stability. Elife. 2020;9:e57277. Published 2020 Aug 14.

Solubility Data

Solubility (In Vitro)

DMSO : ~50 mg/mL (~172.87 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 2.5 mg/mL (8.64 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (8.64 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.4572 mL	17.2861 mL	34.5722 mL
	5 mM	0.6914 mL	3.4572 mL	6.9144 mL
	10 mM	0.3457 mL	1.7286 mL	3.4572 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.