CB2R PAM is an orally bioactive PAM (positive allosteric modulator) of cannabinoid type 2 receptors (CB2Rs). CB2R PAMs display antinociceptive activity in a mouse model of neuropathic pain.

Physicochemical Properties

Molecular Formula	C21H24BRFN2O2
Molecular Weight	435.33
Exact Mass	434.1
CAS #	2244579-87-9
PubChem CID	137553165
Appearance	White to off-white solid powder
Density	1.4±0.1 g/cm3
Boiling Point	572.4±50.0 °C at 760 mmHg
Flash Point	300.0±30.1 °C
Vapour Pressure	0.0±1.6 mmHg at 25°C
Index of Refraction	1.605
LogP	4.78
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	4
Heavy Atom Count	27
Complexity	630
Defined Atom Stereocenter Count	0
InChi Key	NSGDYZCDUPSTQT-UHFFFAOYSA-N
InChi Code	InChI=1S/C21H24BrFN2O2/c1-14-18(22)13-25(12-15-8-10-17(23)11-9-15)21(27)19(14)24-20(26)16-6-4-2-3-5-7-16/h8-11,13,16H,2-7,12H2,1H3,(H,24,26)
Chemical Name	N-[5-bromo-1-[(4-fluorophenyl)methyl]-4-methyl-2-oxopyridin-3-yl]cycloheptanecarboxamide
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	cannabinoid type-2 receptors
ln Vitro	The capacity of CP55940 and 2-AG, but not AEA, to activate [35S]GTPγS binding to CB2Rs is greatly increased by CB2R PAM (100 nM)[1].
ln Vivo	CB2R PAM exhibits antinociceptive action at doses of 1–20 mg/kg; po[1].
Animal Protocol	Animal/Disease Models: Male CD-1 albino mice (Oxaliplatin-induced neuropathic pain model)[1] Doses: 1, 5, 10, 20 mg/ kg Route of Administration: Po Experimental Results: Dramatically increased licking latency in the animals starting from 5 mg/kg.
References	[1]. Identification of the First Synthetic Allosteric Modulator of the CB2 Receptors and Evidence of Its Efficacy for Neuropathic Pain Relief. J Med Chem. 2019;62(1):276-287.

Solubility Data

Solubility (In Vitro)

DMSO: 50 mg/mL (114.86 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 2.5 mg/mL (5.74 mM) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.2971 mL	11.4855 mL	22.9711 mL
	5 mM	0.4594 mL	2.2971 mL	4.5942 mL
	10 mM	0.2297 mL	1.1486 mL	2.2971 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.