Benzylurea is a benzylamide. Benzylurea can be extracted from the stems of Salvadora persica. Benzylurea has antimicrobial activity. Benzylurea may be utilized in research in a variety of biochemical studies.

Physicochemical Properties

Molecular Formula	C8H10N2O
Molecular Weight	150.18
Exact Mass	150.079
CAS #	538-32-9
PubChem CID	10853
Appearance	White to off-white solid powder
Density	1.138g/cm3
Boiling Point	282.9ºC at 760mmHg
Melting Point	149-151 °C(lit.)
Flash Point	124.9ºC
Index of Refraction	1.565
LogP	1.946
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	1
Rotatable Bond Count	2
Heavy Atom Count	11
Complexity	130
Defined Atom Stereocenter Count	0
InChi Key	RJNJWHFSKNJCTB-UHFFFAOYSA-N
InChi Code	InChI=1S/C8H10N2O/c9-8(11)10-6-7-4-2-1-3-5-7/h1-5H,6H2,(H3,9,10,11)
Chemical Name	benzylurea
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Benzylamides from Salvadora persica. Arch Pharm Res.

Solubility Data

Solubility (In Vitro)

DMSO : 100 mg/mL (665.87 mM) Ethanol : 50 mg/mL (332.93 mM) H2O : 3.33 mg/mL (22.17 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 5 mg/mL (33.29 mM) (saturation unknown) in 10% EtOH + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear EtOH stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 5 mg/mL (33.29 mM) (saturation unknown) in 10% EtOH + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear EtOH stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 5 mg/mL (33.29 mM) (saturation unknown) in 10% EtOH + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear EtOH stock solution to 900 μL of corn oil and mix well.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	6.6587 mL	33.2934 mL	66.5868 mL
	5 mM	1.3317 mL	6.6587 mL	13.3174 mL
	10 mM	0.6659 mL	3.3293 mL	6.6587 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.