BODIPYFL C12 is a BODIPY dye. BODIPY dye is a small molecule dye with strong UV absorption ability. Its fluorescence peak is relatively sharp and its quantum yield is high. It is relatively insensitive to the polarity and pH of the environment and, therefore, is relatively stable under different physiological conditions. Due to the asymmetry of its structure, BODIPY has a variety of analogue structural products. BODIPY lipid droplet dye can penetrate the cell membrane well into the interior of the cell and perform specific staining by localizing on the polar lipids in the cell. Therefore, the dye may be utilized to label living cells and fixed cells. excitation and emmission maximum (wavelength): 480/508 nm.

Physicochemical Properties

Molecular Formula	C23H32BN2O2F2-.H+
Molecular Weight	418.328
CAS #	158757-79-0
Appearance	Typically exists as solid at room temperature
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro

Guidelines for usage 1. Parts of the working solution for BODIPYFL C12 1.1 Prepare the stock solution. Load 382 μL of anhydrous DMSO with 1 milligram of BODIPYFL C12. Take out the stock solution (10 mM). Note: It is advised to aliquot and store the BODIPYFL C12 storage solution at -20°C or -80°C. 1.2 To substitute the working solution, make a 1–10 μM BODIPYFL C12 working solution using hot serum-free cell culture medium or PBS loading storage solution. ..Note: Please store the BODIPYFL C12 working solution away from light and adjust the concentration based on the actual situation. 2. Suspended cell staining (2.1): Centrifuge cells, add PBS, then wash twice for five minutes each time. Add 1 mL of BODIPYFL C12 working solution when the cell density reaches 1×106/mL 2.2. Then, follow the flow chart for 5–30 minutes. 2.3 Centrifuge for 3–4 minutes at 400 g, then remove the supernatant. 2.4 Wash the cells twice with PBS, giving them five minutes each time. 2.5 Utilize a fluorescent microscope 3 after resuspending the cells in 1 mL of serum-free or PBS. Adherent cell staining: 3.1 Grow the adherent cells on sterile coverslips. 3.2 Aspirate a tiny quantity of culture medium after removing the coverslip from the medium. 3.3 Pour in 100 μL 3.4 Remove the dye working solution, rinse two or three times in culture media for five minutes each time, and use a flow cytometer or fluorescence microscope to observe. Storage requirements: -20°C, one year of light protection. Observations 1. Please modify the smoking time and BODIPYFL C12 working fluid concentration based on the current circumstances. 2. It is advised to perform a control experiment where liver cancer cells are exposed to an acid oil gradient (30 μM) for eight hours. 3. This product may not be used for clinical diagnosis or treatment, nor for use in food or medicine. It is intended solely for professional use in scientific study. 4. Please put on a lab coat and disposable operating gloves for your own health and safety.

References

[1]. Fluopack screening platform for unbiased cellular phenotype profiling. Sci Rep. 2020 Feb 7;10(1):2097. [2]. BODIPY dyes in photodynamic therapy. Chem Soc Rev. 2013 Jan 7;42(1):77-88. [3]. BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry. Bio Protoc. 2016 Sep 5;6(17):e1912. [4]. Importance of cholesterol-rich membrane microdomains in the interaction of the S protein of SARS-coronavirus with the cellular receptor angiotensin-converting enzyme 2. Virology. 2008 Nov 25;381(2):215-21.

Solubility Data

Solubility (In Vitro)

May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples

Solubility (In Vivo)

Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples. Injection Formulations (e.g. IP/IV/IM/SC) Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] *Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline) Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline) Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline) Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil) Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Oral Formulations Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). Oral Formulation 3: Dissolved in PEG400 Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose Oral Formulation 6: Mixing with food powders Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.3905 mL	11.9523 mL	23.9046 mL
	5 mM	0.4781 mL	2.3905 mL	4.7809 mL
	10 mM	0.2390 mL	1.1952 mL	2.3905 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.