BODIPY493/503 is a BODIPY dye. BODIPY dye is a small molecule dye with strong UV absorption ability. Its fluorescence peak is relatively sharp and its quantum yield is high. It is relatively insensitive to the polarity and pH of the environment and, therefore, is relatively stable under different physiological conditions. Due to the asymmetry of its structure, BODIPY has a variety of analogue structural products. BODIPY lipid droplet dye can penetrate the cell membrane well into the interior of the cell and perform specific staining by localizing on the polar lipids in the cell. Therefore, the dye may be utilized to label living cells and fixed cells. excitation and emmission maximum (wavelength): 493/503 nm.

Physicochemical Properties

Molecular Formula	C14H17BF2N2
Molecular Weight	262.1060
Exact Mass	262.145
CAS #	121207-31-6
PubChem CID	14766991
Appearance	Brown to reddish brown solid powder
Melting Point	257 °C
LogP	2.826
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	0
Heavy Atom Count	19
Complexity	505
Defined Atom Stereocenter Count	0
InChi Key	DRJHPEGNOPSARR-UHFFFAOYSA-N
InChi Code	InChI=1S/C14H17BF2N2/c1-8-6-10(3)18-13(8)12(5)14-9(2)7-11(4)19(14)15(18,16)17/h6-7H,1-5H3
Chemical Name	2,2-difluoro-4,6,8,10,12-pentamethyl-3-aza-1-azonia-2-boranuidatricyclo[7.3.0.03,7]dodeca-1(12),4,6,8,10-pentaene
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro

How to apply 1. 1.1 Preparation of stock solution: Dissolve 1 milligram of BODIPY 493/503 in 382 μL DMSO to create a 10 mM stock solution. This completes the preparation of the working solution. Note: It is advised to keep the stock solution between -20°C and -80°C in storage. To obtain a 1-10 μM working solution, do not use 1.2 BODIPY 493/503 working solution without bone marrow or PBS extraction solution. Note: Please modify the working solution for BODIPY 493/503 based on the current circumstances. 2. Suspension cells (6-well plate) 2.1 Cell staining a. Discard the supernatant after centrifuging at 1000g for three to five minutes at 4°C. Use PBS to wash twice, for five minutes each. There is b cell density. For five to thirty minutes, add one milliliter of the working solution. 1×106/mL. Discard the supernatant after centrifuging at 400 g for three to four minutes at 4°C. d. Use PBS to wash twice, for five minutes each time. e. Re-suspend the cells in PBS or serum-free cells. 2.2 Adherent cells as observed using a flow cytometer or fluorescence microscopy a. On sterile coverslips, cultivate adherent cells. b. Empty the coverslip of any extra cells by removing it from the culture medium. C. To fully cover it, add 100 μL of working solution and give it a little shake. Prelude: 5–30 minutes for cells. d. Use water to wash twice, for five minutes each time. observation using flow cytometry or fluorescence microscopy. For a year, store at -20°C; shield from light. Observations 1. Kindly modify the BODIPY 493/503 working fluid concentration based on the current circumstances. 2. Prior to conducting the next studies, Saudi cells were first cultured with 30 μM oleic acid for eight hours. 3. The creator created and utilized this product; it is not intended for use as a home or medical tool. 4. When working, please use a lab coat and disposable gloves for your own health and safety.

References

[1]. BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry. Bio Protoc. 2016 Sep 5;6(17):e1912.

Solubility Data

Solubility (In Vitro)

DMSO : ~5 mg/mL (~19.08 mM)

Solubility (In Vivo)

Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples. Injection Formulations (e.g. IP/IV/IM/SC) Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] *Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline) Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline) Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline) Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil) Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Oral Formulations Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). Oral Formulation 3: Dissolved in PEG400 Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose Oral Formulation 6: Mixing with food powders Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.8152 mL	19.0760 mL	38.1519 mL
	5 mM	0.7630 mL	3.8152 mL	7.6304 mL
	10 mM	0.3815 mL	1.9076 mL	3.8152 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.