Physicochemical Properties
| Molecular Formula | C223H417N89O50S |
| CAS # | 2232156-31-7 |
| Appearance | Typically exists as solid at room temperature |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| ln Vitro | Apoptin-derived peptide (40-100 μg/mL, 24 h) reverses the resistance of SGC-7901/CDDP cells to CDDP via promoting ARNT downregulation through inhibition of the PI3K/AKT signaling pathway [1]. The migration and invasion of SGC-7901, SGC-7901/CDDP, and MGC-803 cells are inhibited by apoptin-derived peptide (10–30 μg/mL, 24 h) [1]. The sensitivity of SGC-7901/CDDP cells to CDDP is increased by apoptin-derived peptide (30–60 μg/mL, 24 h) [1]. The Apoptin-derived peptide (10–20 μg/mL, 24 h) causes apoptosis and raises the percentage of G2/M phase cells in SGC-7901/CDDP cells [1]. The gastric cancer cells are effectively inhibited by apoptin-derived peptide (10-70 μg/mL, 4-24 h) [1]. |
| Cell Assay |
Cell Viability Assay[1] Cell Types: HEK293, SGC-7901, SGC-7901/CDDP Tested Concentrations: 10 μg/mL, 20 μg/mL, 30 μg/mL, 40 μg/mL, 50 μg/mL, 60 μg/ mL, 70 μg/mL Incubation Duration: 4 h, 6 h, 8 h, 12 h, 18 h, 24 h Experimental Results: Produced large effects in both SGC-7901 and SGC-7901/CDDP cells at 60 μg/mL for 6 h and had no obvious effect on HEK293 cells. Apoptosis Analysis[1] Cell Types: SGC-7901, SGC-7901/CDDP Tested Concentrations: 30 μg/mL, 60 μg/mL Incubation Duration: 24 h Experimental Results: Induced SGC-7901 cell apoptosis in a concentration dependent manner. Induced considerable apoptotic cell death in SGC-7901/CDDP cells in a strong time- and dose-dependent fashion. Cell Cycle Analysis[1] Cell Types: SGC-7901, SGC-7901/CDDP Tested Concentrations: 10 μg/mL, 20 μg/mL Incubation Duration: 24 h Experimental Results: Increase the G2/M phase population. Cell Invasion Assay[1] Cell Types: SGC-7901, SGC-7901/CDDP, MGC-803 Tested Concentrations: 10 μg /mL, 20 μg/mL, 30 μg/mL Incubation Duration: 48 h Experimental Results: Dramatically diminished the number of invasive cells. Cell Migrat |
| References |
[1]. Apoptin-derived peptide reverses cisplatin resistance in gastric cancer through the PI3K-AKT signaling pathway . Cancer Medicine, 2018, 7(4): 1369-1383. |
Solubility Data
| Solubility (In Vitro) | May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples |
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples. Injection Formulations (e.g. IP/IV/IM/SC) Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] *Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline) Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline) Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline) Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil) Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Oral Formulations Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). Oral Formulation 3: Dissolved in PEG400 Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose Oral Formulation 6: Mixing with food powders Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently. (Please use freshly prepared in vivo formulations for optimal results.) |