AT-9010 tetrasodium is the triphosphate bioactive metabolite of AT-527 and is a potent inhibitor of NiRAN (a function essential for viral replication). AT-9010 tetrasodium can inhibit the replication of SARS-CoV-2.

Physicochemical Properties

Molecular Formula	C11H13FN5NA4O13P3
Molecular Weight	627.13
Exact Mass	626.929
CAS #	1621884-18-1
Related CAS #	AT-9010;1261253-79-5;AT-9010 triethylamine;2648089-95-4
PubChem CID	166638290
Appearance	White to off-white solid powder
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	16
Rotatable Bond Count	7
Heavy Atom Count	37
Complexity	952
Defined Atom Stereocenter Count	4
SMILES	C[C@]1([C@@H]([C@@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)O[C@H]1N1C=NC2C(N=C(N)NC1=2)=O)O)F.[Na+].[Na+].[Na+].[Na+]
InChi Key	YSFIWHKJCYGPPP-ZJECJYFYSA-J
InChi Code	InChI=1S/C11H17FN5O13P3.4Na/c1-11(12)6(18)4(2-27-32(23,24)30-33(25,26)29-31(20,21)22)28-9(11)17-3-14-5-7(17)15-10(13)16-8(5)19;;;;/h3-4,6,9,18H,2H2,1H3,(H,23,24)(H,25,26)(H2,20,21,22)(H3,13,15,16,19);;;;/q;4*+1/p-4/t4-,6-,9-,11-;;;;/m1..../s1
Chemical Name	tetrasodium;[[[(2R,3R,4R,5R)-5-(2-amino-6-oxo-1H-purin-9-yl)-4-fluoro-3-hydroxy-4-methyloxolan-2-yl]methoxy-oxidophosphoryl]oxy-oxidophosphoryl] phosphate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage.(2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	NiRAN[1]
ln Vitro	Normal human bronchial and nasal epithelial cells treated with 10 μM AT-511 (698 μM and 236 μM, respectively) produce significant levels of the active triphosphate metabolite AT-9010, which has a half-life of at least 38 hours [1].
References	[1]. Atea Pharmaceuticals Announces Publication of Preclinical Data Highlighting Potent Activity of AT-527 Against SARS-CoV-2. BOSTON, Feb. 08, 2021. Atea Pharmaceuticals, Inc.

Solubility Data

Solubility (In Vitro)	H2O : 100 mg/mL (159.46 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 100 mg/mL (159.46 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.5946 mL	7.9728 mL	15.9457 mL
	5 mM	0.3189 mL	1.5946 mL	3.1891 mL
	10 mM	0.1595 mL	0.7973 mL	1.5946 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.