A25822B is an azasterol antifungal agent. It may decrease the ratio of unsaturated to saturated fatty acids. The A25822 antibiotic complex consists of seven biologically active factors. A comparative study of these factors determined that factor B possessed the greatest antifungal activity. The minimal inhibitory concentration of A25822B against isolates of Candida albicans was less than 0.3 similar to 5.0 mug/ml, Trichophyton mentagrophytes was inhibited at less than 0.0312 mug/ml. Other pathogenic fungi such as Cryptococcus neoformans, Histoplasma capsulatum, Blastomyces dermatitidis, Sporotrichum schenckii, and Microsporium gypseum were very susceptible to A25822B. Only limited antibacterial activity of A25822B was found.

Physicochemical Properties

Molecular Formula	C28H45NO
Molecular Weight	411.663008451462
Exact Mass	411.35
CAS #	50686-98-1
Related CAS #	50686-98-1
PubChem CID	162092
Appearance	Typically exists as solid at room temperature
LogP	6.569
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	5
Heavy Atom Count	30
Complexity	746
Defined Atom Stereocenter Count	4
SMILES	O[C@H]1CC[C@]2(C)C3CC[C@@]4(C)C(C=3CC[C@H]2C1)=NCCC4C(C)CCC(=C)C(C)C
InChi Key	JZFNKAMRJSGWIF-GQECOPKMSA-N
InChi Code	InChI=1S/C28H45NO/c1-18(2)19(3)7-8-20(4)24-13-16-29-26-23-10-9-21-17-22(30)11-14-27(21,5)25(23)12-15-28(24,26)6/h18,20-22,24,30H,3,7-17H2,1-2,4-6H3/t20?,21-,22-,24?,27-,28+/m0/s1
Chemical Name	(6aS,8S,10aS,12aR)-10a,12a-dimethyl-1-(6-methyl-5-methylideneheptan-2-yl)-1,2,3,5,6,6a,7,8,9,10,11,12-dodecahydronaphtho[1,2-h]quinolin-8-ol
Synonyms	A25822B
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	In vitro activity: Kinase Assay: Cell Assay:
ln Vivo
Animal Protocol
References	J Antibiot (Tokyo).1975 Feb;28(2):112-7.

Solubility Data

Solubility (In Vitro)	DMSO: Water: Ethanol:
Solubility (In Vivo)	(Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.4292 mL	12.1459 mL	24.2919 mL
	5 mM	0.4858 mL	2.4292 mL	4.8584 mL
	10 mM	0.2429 mL	1.2146 mL	2.4292 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.