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A 20832 is a potent inhibitor that inhibits resolvase-promoted site-specific recombination postsynaptically at strand cleavage. It does not inhibit resolvase binding to res, as measured by filter binding, or synapse formation. Inhibition of resolvase-promoted site-specific recombination by A20832 occurs postsynaptically at strand cleavage. DNase I analysis in the presence of A20832 indicates that only site I of res is bound by resolvase.
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Physicochemical Properties
| Molecular Formula |
C10H14O3CL2
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| Molecular Weight |
253.122
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| Exact Mass |
252.032
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| CAS # |
40526-96-3
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| Related CAS # |
|
| PubChem CID |
162016
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.202g/cm3
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| Boiling Point |
332.2ºC at 760 mmHg
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| Flash Point |
131ºC
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| Index of Refraction |
1.477
|
| LogP |
2.853
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
6
|
| Heavy Atom Count |
15
|
| Complexity |
280
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C(C(C/C(/C)=C(\Cl)/Cl)C(OCC)=O)(=O)C
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| InChi Key |
CSIXHPCPUJVRQT-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C10H14Cl2O3/c1-4-15-10(14)8(7(3)13)5-6(2)9(11)12/h8H,4-5H2,1-3H3
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| Chemical Name |
ethyl 2-acetyl-5,5-dichloro-4-methylpent-4-enoate
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| Synonyms |
|
| HS Tariff Code |
2934.99.9001
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| Storage |
Powder-20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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|
Biological Activity
| ln Vitro |
In vitro activity:
Kinase Assay:
Cell Assay: |
|
| ln Vivo |
|
| Animal Protocol |
|
| References |
J Biol Chem.1989 Oct 5;264(28):16892-6.
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Solubility Data
| Solubility (In Vitro) |
|
| Solubility (In Vivo) |
(Please use freshly prepared in vivo formulations for optimal results.)
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| Preparing Stock Solutions |
|
1 mg |
5 mg |
10 mg |
| 1 mM |
3.9507 mL |
19.7535 mL |
39.5070 mL |
| 5 mM |
0.7901 mL |
3.9507 mL |
7.9014 mL |
| 10 mM |
0.3951 mL |
1.9753 mL |
3.9507 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles. |
