7-Methylguanine is a metabolite of DNA methylation. It can be generated by methylation reagents and used as a probe for protein-DNA interactions and a key component of DNA sequencing methods.

Physicochemical Properties

Molecular Formula	C6H7N5O
Molecular Weight	165.1527
Exact Mass	165.065
CAS #	578-76-7
Related CAS #	7-Methylguanine-d3;17829-03-7
PubChem CID	135398679
Appearance	White to light yellow solid powder
Density	1.8±0.1 g/cm3
Boiling Point	481.4±37.0 °C at 760 mmHg
Melting Point	370°C
Flash Point	244.9±26.5 °C
Vapour Pressure	0.0±1.3 mmHg at 25°C
Index of Refraction	1.865
LogP	-2.08
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	0
Heavy Atom Count	12
Complexity	250
Defined Atom Stereocenter Count	0
InChi Key	FZWGECJQACGGTI-UHFFFAOYSA-N
InChi Code	InChI=1S/C6H7N5O/c1-11-2-8-4-3(11)5(12)10-6(7)9-4/h2H,1H3,(H3,7,9,10,12)
Chemical Name	2-amino-7-methyl-1H-purin-6-one
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. Synthesis and structure of duplex DNA containing the genotoxic nucleobase lesion N7-methylguanine. J Am Chem Soc. 2008 Sep 3;130(35):11570-1.
Additional Infomation	2-amino-7-methyl-7H-purin-6-ol is a 7-methylguanine that is 7H-purine substituted by an amino group at position 2, a methyl group at position 7 and a hydroxy group at position 6. It is a tautomer of a 2-amino-7-methyl-1,7-dihydro-6H-purin-6-one and a 2-imino-7-methyl-1,2,3,7-tetrahydro-6H-purin-6-one. 7-Methylguanine is a metabolite found in or produced by Saccharomyces cerevisiae.

Solubility Data

Solubility (In Vitro)

DMSO : ~50 mg/mL (~302.76 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (15.14 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	6.0551 mL	30.2755 mL	60.5510 mL
	5 mM	1.2110 mL	6.0551 mL	12.1102 mL
	10 mM	0.6055 mL	3.0276 mL	6.0551 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.