6-Methylnicotinamide is an analogue of nicotinamide and an endogenously produced metabolite. During the acute phase of intracerebral hemorrhage (ICH), sudden increases in 6-Methylnicotinamide can exacerbate neurological damage.

Physicochemical Properties

Molecular Formula	C7H8N2O
Molecular Weight	136.1512
Exact Mass	136.063
CAS #	6960-22-1
PubChem CID	96351
Appearance	White to off-white solid powder
Density	1.2±0.1 g/cm3
Boiling Point	289.4±28.0 °C at 760 mmHg
Melting Point	197-199 °C(lit.)
Flash Point	128.8±24.0 °C
Vapour Pressure	0.0±0.6 mmHg at 25°C
Index of Refraction	1.561
LogP	0.35
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	1
Heavy Atom Count	10
Complexity	136
Defined Atom Stereocenter Count	0
InChi Key	IJXDURUAYOKSIS-UHFFFAOYSA-N
InChi Code	InChI=1S/C7H8N2O/c1-5-2-3-6(4-9-5)7(8)10/h2-4H,1H3,(H2,8,10)
Chemical Name	6-methylpyridine-3-carboxamide
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Temporal metabolomic alteration in rat brains of experimental intracerebral hemorrhage. Brain Res Bull. 2021 May;170:234-245.

Solubility Data

Solubility (In Vitro)

DMSO : ~50 mg/mL (~367.24 mM) H2O : ~3.33 mg/mL (~24.46 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (18.36 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (18.36 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (18.36 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	7.3448 mL	36.7242 mL	73.4484 mL
	5 mM	1.4690 mL	7.3448 mL	14.6897 mL
	10 mM	0.7345 mL	3.6724 mL	7.3448 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.